摘要
以马铃薯品种东农303微型薯为试验材料,通过根癌农杆菌介导法,将来自耐旱植物厚叶旋蒴苣苔的脱水蛋白基因BDN1导入马铃薯,并对其遗传转化的影响因素进行了研究。获得了抗性再生植株35株;PCR检测27株呈阳性;Southern-blot检测4株呈阳性,表明BDN1基因已整合到马铃薯基因组中;半定量RT-PCR检测结果表明BDN1基因在马铃薯抗性植株中表达。通过对相对含水量、脯氨酸、丙二醛含量和SOD活性测定,证明4个转基因株系对干旱胁迫的抗性显著高于对照植株。
The BDN1 gene in coding area from drought-tolerant Boea crassifolia Hemsl was transferred into potato(Solanum tuberosum L.)cultivars' Dongnong 303'through agro-bacteriummediated transformation.The factors influencing its genetic transformation were studied.Thirty-five transgenic plants with kanamycin resistance were obtained.Specific primers were used to amplify the full length of the BDN1 gene from total genomic DNA of the transgenic plants.Among them,27 plants were positive by PCR test and 4 plants were positive by Southern-blot test.The result of semi-quantitative RT-PCR test showed that BDN1 gene was inserted into the genome of the transgenic plants and can be transcribed normally.The physiological index showed the transgenic potato plants were significantly tolerant to drought stress.
出处
《中国蔬菜》
北大核心
2013年第03X期37-43,共7页
China Vegetables
基金
黑龙江省科技计划项目(GZ11B103)
国家高技术研究发展"863"计划项目(2009AA10Z103)
关键词
马铃薯
农杆菌介导
BDN1基因
抗旱
遗传转化
Potato
Agrobacterium-mediated
BDN1 gene
Drought-tolerant
Genetic transformation
