摘要
目的通过建立外阴阴道假丝酵母菌病(vulvovaginal candidiasis,VVC)大鼠模型来评估水溶性壳聚糖妇科软凝胶(商品名:富宁康)治疗VVC的抗真菌及黏膜修复作用。方法 2018年9-12月于首都医科大学附属北京妇产医院进行相关动物实验。将30只雌性Sprague Dawley(SD)大鼠分别随机分为正常对照组、感染模型组、富宁康组、制霉菌素组及雌激素组,根据分组连续给药7 d,停药后取阴道分泌物进行检查,计算病原体转阴率,取阴道标本进行苏木精-伊红染色法(hematoxylin-eosin,HE)、免疫组化及透射电镜检测阴道组织变化。结果停药1 d及4 d后富宁康的病原体转阴率均为100%。富宁康治疗后,大鼠阴道黏膜厚度较感染大鼠显著增加[(114.15±12.65)μm vs.(74.47±28.90)μm,P=0.001],阴道上皮结构基本恢复至正常。此外,富宁康治疗后白细胞介素(interleukin,IL)-4及干扰素(interferon,IFN)-γ未见显著改变,但IL-17(P=0.035)及非B细胞Ig G(P=0.003)表达上调。结论富宁康能够有效清除真菌,修复阴道黏膜并调节局部固有免疫应答。
Objective To establish the rat model of vulvovaginal candidiasis(VVC)and evaluate the antifungal and mucosal repair effects of water-soluble chitosan gynecological soft gel(Funingkang)on VVC.Methods From September 2018 to December 2018,animal experiments were conducted in Beijing Obstetrics and Gynecology Hospital,Capital Medical University.Thirty female Sprague Dawley(SD)rats were randomized into five treatment groups:control,infection model,Funingkang,nystatin and estrogen groups.The drug was administered continuously for 7 days according to the grouping.Vaginal secretions were collected after treatment and the clearance rate of pathogens was calculated.Vaginal specimens were assessed by hematoxylin-eosin(HE)staining,immunohistochemistry and transmission electron microscopy.Results The negative conversion rate of pathogen in Funingkang group was 100%(6/6)one day and four days after drug withdrawal.After treatment with Funingkang,the mean thickness of vaginal mucosa was significantly increased compared with that of infected rats[(114.15±12.65)μm vs.(74.47±28.90)μm,P=0.001],and the morphology of vaginal epithelium returned to normal.In addition,the expression of interleukin(IL)-4 and interferon(IFN)-γkept unchanged,but IL-17(P=0.035)and non B-IgG(P=0.003)were up-regulated after treatment with Funingkang.Conclusion Funingkang can effectively eliminate the pathogen of Candida albicans,repair vaginal mucosa,and regulate the innate immune response.
作者
李婷
刘朝晖
张旭
陈曦
王素霞
LI Ting;LIU Zhao-hui;ZHANG Xu;CHEN Xi;WANG Su-xia(Department of Gynecology,Beijing Obstetrics and Gynecology Hospital,Capital Medical University,Beijing 100026,China)
出处
《中国实用妇科与产科杂志》
CAS
CSCD
北大核心
2019年第10期1156-1160,共5页
Chinese Journal of Practical Gynecology and Obstetrics
基金
国家自然科学基金(81771530及81801406)
北京市医管局“青苗”计划专项经费(QML20181402)
