摘要
目的研究医护人员携带的金黄色葡萄球菌耐消毒剂基因及其人群分布情况,为防控感染提供参考。方法采用细菌分离鉴定和聚合酶链反应(PCR)方法,对部分医护人员和健康体检人群鼻前庭棉拭子标本进行检测与分析。结果从110份医护人员标本中检出金黄色葡萄球菌41株,检出率为37. 3%;从336名体检人员中,分离出金黄色葡萄球菌84株,检出率为25. 0%,医护人员金黄色葡萄球菌检出率明显高于体检人员(P=0. 01,P <0. 05)。41株医护人员携带的金黄色葡萄球菌检出6株携带qac G,H基因或J基因,携带率为14. 63%;84株健康体检人员携带的金黄色葡萄球菌检出3株携带qac G,H或J基因,携带率为3. 67%,医护人员携带含耐消毒剂基因的金黄色葡萄球菌明显高于体检人员(P=0. 012,P <0. 05)。所有凝固酶阴性葡萄球菌中并未检测出耐消毒剂基因。结论医护人员携带的金黄色葡萄球菌耐消毒剂基因携带率高于健康志愿者,提示医院环境内消毒剂的广泛应用可能会诱导金黄色葡萄球菌耐消毒剂基因的表达。
Objective To investigate the anti-disinfectant genes of Staphylococcus aureus carried by medical staff and their population distribution,so as to provide reference for prevention and control of infection.Methods Bacterial isolation and polymerase chain reaction(PCR)were used to detect and analyze cotton swab specimens of nasal vestibule collected from some medical staff and healthy people.Results 41 strains of Staphylococcus aureus were detected from 110 medical staff samples,and the detection rate was 37.3%;84 strains of Staphylococcus aureus were isolated from 336 physical examination personnel,and the detection rate was 25.0%.The detection rate of Staphylococcus aureus in medical staff was significantly higher than that of physical examination personnel(P=0.01,P<0.05).6 of 41 strains of Staphylococcus aureus isolated from medical staff carried qac G,H or J gene,and the carrying rate was 14.63%;only 3 of 84 strains of Staphylococcus aureus isolated from healthy volunteers carried qac G,H or J genes,and the carrying rate was 3.67%,the Staphylococcus aureus containing the anti-disinfectant gene in medical staff was significantly higher than that in physical examination personnel(P=0.012,P<0.05).No anti-disinfectant gene was detected in all coagulase-negative staphylocci.Conclusion The carrying rate of Staphylococcus aureus containing anti-disinfectant genes in medical staff is higher than that of healthy volunteers,suggesting that the widespread use of disinfectants in hospital environments may induce the expression of Staphylococcus aureus anti-disinfectant genes.
作者
叶慧芬
曾俊韶
覃文周
YE Hui-fen;ZENG Jun-shao;QIN Wen-zhou(Guigang People's Hospital of Guangxi,Guigang Guangxi 537100,China)
出处
《中国消毒学杂志》
CAS
2019年第10期748-750,共3页
Chinese Journal of Disinfection
