摘要
关于丹参的干燥方式古今研究尚未达成共识,新鲜丹参中酚酸成分的含量还存在较大争议,为进一步探明新鲜丹参中主要成分的含量及其干燥过程中的动态变化规律,该实验以主要成分含量为评价指标,对新鲜丹参的处理方式、干燥方法、脱水率与成分含量的相关性等进行研究,并在丹参干燥过程中设置密封与不密封的平行对照进行验证试验。采用UPLC波长切换法测定丹参中迷迭香酸、紫草酸、丹酚酸B、隐丹参酮、丹参酮Ⅰ、亚甲基丹参醌、丹参酮ⅡA7种主要成分的含量,结果显示,新鲜丹参中酚酸成分的含量较低,干燥过程中随着脱水增加,酚酸成分含量显著增加,而丹参酮成分的含量变化不大;3种干燥方法,50℃烘干优于晒干,晒干优于阴干,50℃烘干为丹参的最佳干燥方法。通过验证试验和SPSS软件分析丹参脱水与酚酸成分含量的相关性,进一步证明了脱水率与酚酸成分的含量呈显著正相关。该研究为丹参药材产地加工及干燥方法提供参考,对提升丹参质量与品质具有重要意义。
There is no consensus on the drying methods of Salvia miltiorrhiza in ancient and modern times,especially on the content of phenolic acid in fresh S.miltiorrhiza.In order to further explore the content of main components in fresh S.miltiorrhiza and study the dynamic changes during the drying process,the content of main components was used as the index in this study to evaluate the processing method,drying method,correlation between dehydration rate and component content for fresh S.miltiorrhiza.In addition,the sealed and unsealed parallel control groups were set to carry out verification test during the drying process.UPLC method was used for determination of seven main components including rosmarinic acid,lithosperic acid,salvianolic acid B,cryptotanshinone,tanshinoneⅠ,methylene salianolate and tanshinoneⅡAin S.miltiorrhiza.The results showed that the fresh S.miltiorrhiza contained low levels of phenolic acid,and the content of phenolic acid increased significantly with the increase of dehydration rate during drying process,while the change of tanshinone was not obvious.In the comparison of three drying methods,we found that drying at 50℃was better than drying in the sun,and drying in the sun was superior to drying in the shade.So,drying at 50℃was the best drying method.The correlation between dehydration and phenolic acid content of S.miltiorrhiza was analyzed by verification test and SPSS software,which further proved that the dehydration rate was significantly positively correlated with the content of phenolic acid components.This study provides reference for the production processing and drying methods of S.miltiorrhiza medicinal materials,which is of great significance for improving the quality of S.miltiorrhiza.
作者
周涛
罗春梅
张松林
黄志芳
汤依娜
刘玉红
刘云华
陈燕
李青苗
易进海
ZHOU Tao;LUO Chun-mei;ZHANG Song-lin;HUANG Zhi-fang;TANG Yi-na;LIU Yu-hong;LIU Yun-hua;CHEN Yan;LI Qing-miao;YI Jin-hai(Sichuan Provincial Key Laboratory of Quality and Innovation Research of Chinese Materia Medica,Sichuan Academy of Chinese Medicine Sciences,Chengdu 610041,China;School of Pharmacy of Southwest Medical University,Luzhou 646000,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2019年第21期4634-4640,共7页
China Journal of Chinese Materia Medica
基金
国家“重大新药创制”科技重大专项(2018ZX09731-010,2018ZX09201018-029)
国家中药标准化项目(ZYBZH-Y-SC-40)
关键词
丹参
干燥
化学成分
含量
动态变化
UPLC
Salvia miltiorrhiza
drying
chemical composition
content
dynamic change
UPLC
