摘要
目的:应用顺序特异性引物聚合酶链反应技术(PCR-SSP)进行临床肾移植供受者HLA-DR位点DNA的分型。方法:设计并合成HLA-DR位点16对特异性引物和1对阳性对照引物,建立PCR-SSP法,对52份临床肾移植供受者的外周血淋巴细胞样本进行DR位点基因的分型。结果与微量PCR-SSP基因分型试剂盒分型方法比较。结果:所有临床样本的PCR-SSP基因分型均获得成功,结果与微量PCR-SSP基因分型试剂盒分型方法完全相同,分型时间3 h,特异性和重复性100％。结论:应用合成引物为临床肾移植供受者进行HLA-DR位点PCR-SSP基因分型简便快捷,重复性好,适合于临床应用。
AIM: To type the HLA-DR DNA for renal transplantation by PCR with sequence-specific primers (PCR-SSP). METHODS: According to nucleotide sequences of HLA-DR, 16 pairs of specific primers and a pair of positive control primers were designed and synthesized for PCR-SSP. Then HLA-DR sites of 52 donors and recipients for renal transplantation were typed by the PCR-SSP. RESULTS: All the samples were successfully typed by PCR-SSP with the synthesized primers. The results were available within 3 hours after sampling and the accuracy and reproducibility were 100%. CONCLUSION: Genotyping for HLA-DR sites by PCR-SSP with primers reported herein was a simple and accurate technique suitable for clinical application.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2003年第6期560-562,共3页
Chinese Journal of Cellular and Molecular Immunology
