摘要
目的测定吴茱萸生药和提取物中总皂苷、总黄酮和总酚酸的含量.方法采用80%乙醇提取,正丁醇萃取,乙醚脱脂的方法提取吴茱萸;用分光光度法测定总皂苷、总黄酮和总酚酸的含量.结果实验用的两种吴茱萸生药中总皂苷、总黄酮和总酚酸的百分含量分别为5.78、5.24、30.23和12.36、10.99、24.36;提取物中总皂苷、总黄酮和总酚酸的百分含量分别为29.56、52.14、81.88.结论不同吴茱萸药材中总皂苷、总黄酮和总酚酸的含量差异显著(t检验,P<0.01);吴茱萸提取物中含有大量的皂苷类、黄酮类和酚酸类成分.
Objective To determine contents of total saponins, flavonoids and total phenolic acid in Evodia rutaecarpa(Juss.)Benth and its extractive.Method The extractive was produced through extracting by 80% ethanol and normal butanol,degreasing by Ether.Total saponins,flavonoids and total phenolic acid was determined by spectrophotometry.Resutlt The percent contents of total saponins,flavonoids and total phenolic acid in two kinds of Evodia rutaecarpa(Juss.)Benth were 5.78,5.24,30.23 and 12.36,10.99,24.36,in the extractive were 29.56,52.14,81.88,respectively.Conclusion Contents of total saponins,flavonoids and total phenolic acid were notably different in different Evodia rutaecarpa(Juss.)Benth(t test,P<0.01).There was a lot of ingredient of saponins,flavonoids and phenolic acid in the extractive.
出处
《河北北方学院学报(自然科学版)》
2008年第A03期27-29,33,共4页
Journal of Hebei North University:Natural Science Edition
关键词
吴茱萸
提取物
总皂苷
总黄酮
总酚酸
Evodia rutaecarpa(Juss.)Benth
extractive
total saponins
total flavonoids
total phenolic acid