摘要
目的:探讨重组稳定表达狂犬病毒糖蛋白的新城疫病毒疫苗(recombinant avirulent newcastle disease virus LaSota strain expressing the rabies virus glycoprotein,rl-RVG)对人肺腺癌A549细胞荷瘤鼠的生长抑制作用及其可能的免疫机制。方法:建立A549荷瘤鼠模型并随机分为rl-RVG组、新城疫病毒(NDV)组和对照组,每组10只,分别于瘤体注射rl-RVG,NDV和PBS,每周2次,共3周。测量3组瘤体体积;免疫组织化学法测NDV及狂犬病毒糖蛋白(rabies virus glycoprotein,RVG)的表达;HE染色观察荷瘤小鼠的瘤体、脾、肺等组织病理改变;流式细胞术检测荷瘤小鼠脾脏NK细胞数。结果:与对照组相比,rl-RVG组及NDV组荷瘤鼠瘤体增长缓慢;NDV在rl-RVG组和NDV组中均有表达,而RVG仅在rl-RVG组中表达;rl-RVG组及NDV组瘤体出现大量凋亡坏死组织、脾脏多核巨细胞明显增多、肺组织炎症反应明显;rl-RVG组和NDV组NK细胞数明显增多,且rl-RVG组明显高于NDV组。结论:rl-RVG成功转染至A549荷瘤鼠,对其瘤体生长有着明显的抑制作用,可能与rl-RVG激活机体的免疫反应相关。
Objective:To explore the effects of recombinant avirulent newcastle disease virus LaSota strain expressing the rabies virus glycoprotein (rl-RVG) on the proliferation of A549 lung adenocarcinoma tumor-bearing mice and potential immune mechanism.Methods:A tumor model of human A549 into nude mice was constructed and divided randomly into rl-RVG group,newcastle disease virus (NDV) group and control group.The mice in each group received rl-RVG,NDV and PBS injection,respectively,twice a week,for 3 weeks.The growth of tumor were recorded,and the expression of NDV and rabies virus glycoprotein (RVG) were assayed by immunohistochemistry.The pathological change of the tissues were observed by HE staining.The number of NK (CD3-CD49 +) cells were detected by flow cytometry.Results:Compared with the control group,the growth of tumor in rl-RVG group and NDV group were inhibited more effectively.The subcutaneous tumor necrosis were more evident and there were much more multinucleated giant cells in the spleen tissue of the mice in rl-RVG group and NDV group.Immunohistochemical analysis showed that RVG protein was expressed in rl-RVG group and NDV protein was expressed in both rl-RVG group and NDV group.The number of NK cells was significantly higher in rl-RVG group and NDV group than that in the control group,and in rl-RVG group it was more higher than that in NDV group.Conclusion:Recombinant rl-RVG transfected successfully,and effectively inhibited the growth of A549 lung adenocarcinoma by mechanism which activating cell immune response.
出处
《江苏大学学报（医学版）》
CAS
2014年第2期105-109,113,共6页
Journal of Jiangsu University：Medicine Edition
基金
镇江市社会发展基金资助项目(2013041)
