摘要
目的:研究肺腺癌转移相关转录子-1(metastasis-associated lung adenocarcinoma transcript 1,MALAT-1)和核因子-κB(NF-κB)在食管鳞状细胞癌(鳞癌)组织中的表达及其相关性。方法:采用qRT-PCR技术检测60例食管鳞癌组织和癌旁组织中MALAT-1和NF-κB的mRNA水平的表达,免疫组化SP方法检测NF-κB的蛋白表达水平。统计分析MALAT-1及NF-κB与患者临床病理特征之间的关系以及两者的相关性。结果:MALAT-1和NF-κB mRNA在60例食管鳞癌组织中的表达均高于癌旁组织(Z=-3.600,P<0.001;Z=-2.856,P<0.05),且NF-κB蛋白在食管鳞癌组织中的阳性表达率显著高于癌旁组织(χ2=36.39,P<0.001)。MALAT-1、NF-κB的表达均与食管鳞癌的分化程度、淋巴结转移以及TNM分期密切相关(P<0.05)。MALAT-1与NF-κB之间存在正相关(r=0.795,P<0.001)。结论:长链非编码RNA MALAT-1可能在食管鳞癌的发生发展中发挥重要作用。
Objective:To evaluate the expression and correlations of metastasis-associated lung adenocarcinoma transcript 1 (MALAT-1),and NF-κB in the esophageal squamous cell carcinoma (ESCC) tissue.Methods:The expressions of MALAT-1 and NF-κB mRNA were detected by qRT-PCR in 60 ESCC tissues and adjacent non-tumorous tissues.The expression of NF-κB protein was detected by immunohistochemical staining (SP method),and the relationship between MALAT-1 and clinico-pathological feature of ESCC patients was analyzed,and the correlation was explored.Results:The expression of MALAT-1 and NF-κB both were higher in ESCC tissues than that in adjacent non-tumorous tissues (Z =-3.600,P ＜ 0.001 ;Z =-2.856,P ＜0.05).The positive rate of NF-κB protein in ESCC tissues was significantly higher than that in adjacent non-tumor tissues (x2 =36.39,P ＜ 0.001).Meanwhile,the higher expression of MALAT-1 and NF-κB were correlated with the differentiation degree,lymphnode metastasis and clinical stage of ESCC (P ＜ 0.05).And the expression of MALAT-1 and NF-κB was positive correlated (r =0.795,P ＜0.001).Conclusion:MALAT-1 might play a significant role in the occurrence and development of ESCC.
出处
《江苏大学学报（医学版）》
CAS
2014年第2期129-133,共5页
Journal of Jiangsu University：Medicine Edition
基金
镇江市社会发展支撑计划项目(SH2011019
SH2012023)
关键词
肺腺癌转移相关转录子-1
核因子-ΚB
食管鳞状细胞癌
临床病理特征
metastasis-associated lung adenocarcinoma transcript 1
NF-κB
esophageal squamous cell carcinoma
clinicopathological features
