摘要
目的探讨RPL8基因修饰的树突状细胞(DC)对胶质瘤生长的抑制作用。方法通过重组腺病毒将RPL8基因导入树突状细胞,荧光显微镜观察细胞内绿色荧光蛋白的表达,RT-PCR分析细胞内RPL8m RNA表达,RPL8基因修饰的DC与T细胞混合培养,MTT法检测活化T细胞对胶质瘤细胞的杀伤效应。DC注入荷瘤小鼠体内,观察肿瘤体积变化及小鼠生存时间。结果重组病毒转染DC后,细胞内可见绿色荧光,RT-PCR分析细胞内有RPL8m RNA表达;Ad-RPL8组、Ad组及PBS组激活T细胞对GL261细胞的杀伤率分别为78%、49%和43%,Ad-RPL8组较Ad组及PBS组明显增高(P<0.05),RPL8基因修饰的DC注入小鼠后,与Ad组及PBS组比,小鼠生存期明显延长(P<0.05),肿瘤体积变小(P<0.05),肿瘤细胞坏死增多。结论 RPL8基因修饰的DC对胶质瘤具有生长抑制作用,为胶质瘤免疫治疗开辟新途径。
Objective To investigate the anti-glioma effect of dendritic cells(DC) modified by RPL8 gene. Methods DC were infected with the recombinant adenovirus Ad-RPL8. Green fluorescent protein was detected by fluorescence microscopy. Expression of RPL8 m RNA in DC was analyzed by RT-PCR. After the treatment with DC modified by RPL8 gene, the suppression of T cells on GL261 glioma cells was detected by MTT assay. The life span of mice and the pathology morphology of the tumor were analyzed after the mice were injected with DC. Results After infection with Ad-RPL8, green fluorescent and RPL8 m RNA expression were showed. T cells inhibited the growth of GL261 cells after stimulated by DC modified by RPL8 gene. The inhibition rate of T cells on GL261 cells were 78%, 49% and 43% in Ad-RPL8, Ad and PBS group, respectively. The inhibition rate was higer in Ad-RPL8 group than that in Ad and PBS group(P<0.05). The life span of Ad-RPL8 group was significantly prolonged and tumor volume was decreased, compared with Ad and PBS group(P<0.05). Conclusion The DC modified by RPL8 gene could activate T cells to suppress the growth of glioma, which could provide a new method for immunological therapy on glioma.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2015年第1期19-22,共4页
Cancer Research on Prevention and Treatment
基金
贵州省科学技术基金(黔科合丁字20082198)
遵义市科技计划基金(遵义市科合社字201473)
