摘要
目的研究汉防己甲素(Tetrandrine,TET)对人前列腺癌细胞PC3增殖与凋亡的影响及可能的作用机制。方法用不同浓度TET(0、1、2、4、6、8、10μg/ml)处理PC3细胞,锥虫蓝拒染法和MTT法检测TET对PC3细胞生长的影响,流式细胞仪检测TET对PC3细胞凋亡的诱导作用,Western blot法检测TET对细胞凋亡相关蛋白Caspase-3、PARP表达的影响。结果 2μg/ml TET作用24 h对PC3细胞生长的抑制率为(20.96±1.72)%,随着药物浓度的增加,抑制作用加强,10μg/ml TET作用24 h对PC3细胞生长的抑制率达(89.23±4.12)%。TET能诱导PC3细胞凋亡,并能明显诱导凋亡相关基因Caspase-3的剪切活化及其底物PARP的剪切失活。结论 TET能明显抑制PC3细胞生长,诱导细胞凋亡,该作用与TET诱导细胞Caspase-3剪切激活及PARP剪切失活有关。
Objective To investigate the effect of Tetrandrine(TET) on the proliferation and apoptosis of prostate cancer cells PC3 and the possible mechanism. Methods PC3 cells were treated with different concentration of TET(0, 1, 2, 4, 6, 8, 10μg/ml). Trypan blue exclusion assay and MTT assay were used to detect the inhibition effect of TET on the proliferation of PC3. Cell apoptosis was analyzed by flow cytometry. Expression of caspase3 and PARP protein were detected by Western blot. Results The inhibition rate of PC3 cells after treated with 2 μg/ml TET for 24 h was(20.96±1.72) %, and the inhibition effect was increased with the increase of TET concentration. The inhibition rate accounted for(89.23±4.12)% when treated with 10μg/ml TET for 24 h. TET could also induce the apoptosis of PC3. There was obvious cleavage activation of Caspase-3 and deactivation of PARP after treated with TET. Conclusion TET could inhibit the growth of PC3 cells by inducing typical apoptosis. The mechanism may be related to the cleavage activation of Caspase-3 and deactivation of PARP.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2015年第2期142-144,共3页
Cancer Research on Prevention and Treatment
