摘要
目的:探讨血红素氧合酶1(HO-1)在胰腺癌组织中的表达及临床意义。方法:分别用免疫组化法与Western blot法检测80例胰腺癌患者癌组织及癌旁组中HO-1的表达,通过统计学方法分析HO-1表达与胰腺癌患者临床病理特征及预后间的关系。结果:免疫组化与Western blot结果显示,胰腺癌组织HO-1蛋白的阳性表达率与相对表达量均明显高于癌旁胰腺组织(P<0.05);HO-1在胰腺癌组织的表达与TNM分期、肿瘤分化程度及淋巴结转移明显有关(均P<0.05),而与性别、年龄无明显关系(均P>0.05);Log-rank检验显示,胰腺癌组织中HO-1阳性表达患者中位生存期明显低于其阴性表达患者(25.1个月vs. 36.7个月,P<0.05)。结论:HO-1在胰腺癌组织呈高表达,且与胰腺癌的恶性临床特征及不良预后密切相关,可能成为胰腺癌治疗与研究的新靶点。
Objective:To investigate the expression of heme oxygenase-1(HO-1)in pancreatic cancer tissue and its clinical significance.Methods:The HO-1 expressions in specimens of pancreatic cancer tissue and tumor adjacent tissue from 80 cases of pancreatic cancer were determined by immunohistochemical staining and Western blot analysis,respectively.The relations of HO-1 with the clinicopathologic features and prognosis of pancreatic cancer patients were determined by statistical analyses.Results:The results of immunohistochemical staining and Western blot analysis showed that both the positive expression rate and relative expression levels of HO-1 protein in pancreatic cancer tissue were significantly higher than those in tumor adjacent tissue(both P<0.05).The HO-1 expression in pancreatic cancer tissue was significantly associated with TNM stage,degree of tumor differentiation and lymph node metastasis(allP<0.05),but irrelevant to the sex and age(both P>0.05).Log-rank test showed that the median survival time of patients with HO-1 positive expression was significantly lower than that in patients with its negative expression(25.1 months vs.36.7 months,P<0.05).Conclusion:HO-1 is highly expressed in human pancreatic cancer tissue and is closely related to the malignant clinical characteristics and unfavorable prognosis of pancreatic cancer patients.It may probably become a new target for pancreatic cancer investigation and treatment.
作者
刘海潮
刘少朋
白明辉
苏宝威
LIU Haichao;LIU Shaopeng;BAI Minghui;SU Baowei(The Second Department of Hepatobiliary and Hernia Surgey,Luoyang Central Hospital Affiliated to Zhengzhou University,Luoyang,Henan 471000,China)
出处
《中国普通外科杂志》
CAS
CSCD
北大核心
2019年第9期1088-1094,共7页
China Journal of General Surgery
基金
河南省医学科技攻关计划联合共建基金资助项目（2018020906）
河南省洛阳市市级科技医疗卫生基金资助项目（1820003A）
关键词
胰腺肿瘤
血红素加氧酶-1
肿瘤侵润
预后
Pancreatic Neoplasms
Heme Oxygenase-1
Neoplasm Invasiveness
Prognosis
