摘要
目的:研究枸杞多糖(lycium barbarum polysaccharide,LBP)对肝细胞(LO2)脂肪变性的防治作用及其机制。方法:油酸诱导建立非酒精性脂肪变性肝细胞(non-alcoholic fatty liver disease,NAFLD)模型,分为正常对照组、脂肪变模型组、LBP预防组、LBP治疗组。48 h后光镜下观察细胞内脂质累积情况,并检测细胞内谷氨酸氨基转移酶(alanine aminostransferase,ALT)、门冬氨酸氨基转移酶(aspartate aminostransferase,AST)、甘油三酯(triglyceride,TG)含量,及脂联素与抵抗素的基因及蛋白表达情况。结果:光镜下可见模型组肝细胞内较正常对照组有大量橘红色脂滴(P=0.000),LBP预防组和治疗组与模型组比肝细胞内的脂滴明显减少(P=0.000、P=0.000)。LBP上调脂联素m RNA及蛋白表达(P=0.000、P=0.014),抑制抵抗素m RNA及蛋白的表达(P=0.000、P=0.000),降低肝细胞TG、ALT、AST(P=0.000、P=0.000、P=0.000)。结论:LBP可能通过上调脂联素m RNA及蛋白的表达,下调抵抗素m RNA及蛋白表达,从而有效防治NAFLD。
Objective:To investigate the prevention and treatment effects and to preliminary discuss the action mechanisms of lycium barbarum polysaccharide(LBP)on hepatocyte steatosis model of hepatocyte in vitro. Methods:A model of non-alcoholic fatty liver disease(NAFLD)oleic acid-induced steatosis L-O2 strain hepatocyte(normal adult hepatocytes)was established. The model was divided into normal group,steatosis model group,prevention group and treatment group. The accumulation of lipid droplets in hepatocytes was observed under light microscopy after oil red-O staining after 48 h. The levels of alanine aminostransferase(ALT) aspartate aminostransferase(AST) triglyceride(TG) and the expression of m RNA and protein of adiponectin and resistin were detected.Results:Much more obvious orange lipid droplets in hepatocytes were found in model group after culture than in normal group(P=0.000). Lipid droplets decreased in prevention group and treatment group than in model group(P=0.000;P=0.000). The levels of ALT,AST and TG were decreased(P=0.000;P=0.000;P=0.000);the expressions of m RNA and protein of adiponectin were up-regulated(P=0.000;P=0.014)and the expressions of m RNA and protein of resistin were down-regulated(P=0.000;P=0.000). Conclusion:Induced by expression of m RNA and protein of adiponectin and resistin,NAFLD can be prevented and treated effectively by LBP.
出处
《重庆医科大学学报》
CAS
CSCD
北大核心
2015年第11期1481-1485,共5页
Journal of Chongqing Medical University
基金
重庆市卫生局医学科研资助项目(编号:2011-2-077)
关键词
非酒精性脂肪肝
枸杞多糖
脂联素
抵抗素
治疗
预防
non-alcoholic fatty liver disease
lycium barbarum polysaccharide
adiponectin
resistin
treatment
prevention
