摘要
目的:建立葛根与粉葛的HPLC特征图谱,结合化学模式识别方法对不同产区的葛根与粉葛进行区分,并鉴别葛根产区,为科学评价和有效控制二者的质量提供参考。方法:利用HPLC检测23批葛根或粉葛药材,采用"中药色谱指纹图谱相似度评价系统"(2004A版)软件进行相似度评价,建立共有模式。以其色谱峰信息为数据来源,利用3种化学模式识别方法综合分析葛根和粉葛药材的质量。结果:除了样品S22(陕西产地)之外,不同产区的葛根和粉葛的相似度均> 0.9;说明葛根与粉葛药材的相似度良好,采用特征图谱相似度评价方法无法区分葛根与粉葛药材。利用主成分分析只能鉴别葛根与粉葛;偏最小二乘法-判别分析其模型解释度96.4%,预测度74.6%,能够区分葛根与粉葛,并能区分葛根的产区;系统聚类分析与偏最小二乘法-判别分析结果较为一致。结论:利用化学模式识别方法可快速区分葛根与粉葛,鉴别葛根产区,适用于葛根与粉葛的药材质量评价。
Objective: To establish HPLC specific chromatograms of Puerariae Lobatae Radix ( PLR) and Puerariae Thomsonii Radix ( PTR) ,and make a distinction about their species and different habitats of PLR by chemical pattern recognition,provide reliable methods for scientific evaluation and effective control of their quality. Method: HPLC was employed to determine the contents of chemical ingredients in 23 batches of PLR and PTR. The similarity analyzed with “Similarity Evaluation System for Chromatographic Fingerprint of Chinese Materia Medica”( version of 2004A) ,then a common pattern was established. Based on its chemical fingerprint information,the quality of PLR and PTR was comprehensively analyzed by three kinds of chemical pattern recognition methods. Result: In addition to sample S22 ( from Shaanxi province) ,the similarities of 23 batches of samples were more than 0. 9,which showed that similarity of PLR and PTR was good,this method can not differentiate them. Principal component analysis ( PCA) could only identify PLR and PTR,but partial least squares-discriminant analysis ( PLS-DA) could distinguish PLR from PTR and the producing areas of PLR with model interpretation of 96. 4% and prediction of 74. 6%. The result of hierarchical cluster analysis ( HCA) was consistent with PLS-DA. Conclusion: Chemical pattern recognition method can make a distinction between PLR and PTR,as well as different habitats of PLR;it is suitable for quality control of their medicinal materials.
作者
杨园珍
王雅琪
胡恋琪
焦姣姣
杨明
YANG Yuan-zhen;WANG Ya-qi;HU Lian-qi;JIAO Jiao-jiao;YANG Ming(Jiangxi University of Traditional Chinese Medicine(TCM),Nanchang 330004,China;Chengdu University of TCM,Chengdu 611137,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2019年第4期162-166,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家重点研发计划项目(2017YFC1702904)
国家中药标准化项目(ZYBZH-C-SC-54)
关键词
葛根
粉葛
特征图谱
相似度评价
化学模式识别
大豆苷
3’-羟基葛根素
Puerariae Lobatae Radix
Puerariae Thomsonii Radix
specific chromatograms
similarityevaluation
chemical pattern recognition
daidzin
3'-hydroxypuerarin
