摘要
重组痘苗病毒候选疫苗rVTT-JEVE投入使用之前需要进行本体动物免疫和攻毒保护试验,本研究将重组痘苗病毒rVTT-JEVE进行仔猪免疫接种,分别在体液免疫及细胞免疫水平上评价其免疫效果,并检测攻毒后仔猪体内乙型脑炎病毒载量,分析讨论乙型脑炎重组痘苗病毒候选疫苗rVTT-JEVE对猪体的免疫效果。经2次仔猪免疫接种后,重组痘苗病毒rVTT-JEVE组的IgG抗体水平是商品疫苗SA-14-14-2组的1.5倍,显著高于SA-14-14-2组(P<0.05),而且rVTT-JEVE组的中和抗体效价达到SA-14-14-2组的1.7倍,显著高于SA-14-14-2组(P<0.05)。rVTT-JEVE组的T淋巴细胞刺激指数为SA-14-14-2组的1.6倍,显著高于SA-14-14-2组(P<0.05)。本研究结果显示,rVTT-JEVE候选疫苗组和SA-14-14-2商品疫苗组JEV病毒载量均显著低于PBS对照组(P<0.05),均可有效保护仔猪感染JEV。
Recombinant vaccinia virus candidate vaccine rVTT-JEVE needs to carry out ontology animal immunology and protection research challenged with JEV before used.In this study,the recombinant vaccinia virus rVTT-JEVE was used for immunization in piglets.The immune effects of rVTT-JEVE were evaluated at humoral and cellular levels,and viral loads in piglets after challenged with JEV.After immunized twice in piglets,the IgG antibody level of the recombinant vaccinia virus rVTT-JEVE group was 1.5times higher than that the licensed vaccine SA-14-14-2 group(P<0.05).The neutralizing antibody titer of the rVTT-JEVE group was 1.7-fold higher than that of the SA-14-14-2group(P<0.05).T-lymphocyte stimulation index of rVTT-JEVE group was 1.6times higher than that of SA-14-14-2group(P<0.05).The results showed that the viral load of JEV in the rVTT-JEVE candidate vaccine group and the SA-14-14-2licensed vaccine group were significantly lower than that in the PBS control group(P<0.05),and both of them could effectively protect the piglet from infecting JEV.
作者
肖朋朋
刘昊
靖杰
韩继成
曹亮
李成辉
张金勇
李一权
鲁会军
金宁一
XIAO Peng-peng;LIU Hao;JING Jie;HAN Ji-cheng;CAO Liang;LI Cheng-hui;ZHANG Jin-yong;LI Yi-quan;LU Hui-jun;JIN Ning-yi(Institute of Virology,Wenzhou University,Wenzhou,Zhejiang 325035,China;Military Veterinary Institute,Academy of Military Medical Science,Changchun 130122,China;Institute of Special Economic Animal and Plant Sciences,Chinese Academy of Agricultural Sciences,Changchun 130122,Chin;College of Veterinary Medicine,Jilin University,Changchun 130062,China;College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China)
出处
《中国兽医学报》
CAS
CSCD
北大核心
2019年第2期244-249,共6页
Chinese Journal of Veterinary Science
基金
国家重点研发计划资助项目(2016YFD0500401)
公益性行业(农业)科研资助项目(201203082)
