摘要
为了探明石斛内生细菌DEB-2菌株对贵州辣椒病原真菌的抑制作用,采用生长速率法测定了菌株DEB-2发酵液对辣椒黑斑病病原菌Alternaria alternate、辣椒疫病病原菌Phytophthora capsici、辣椒黑点炭疽病病原菌Colletotrichum capsici、辣椒红色炭疽病病原菌Colletotrichum gloesporioide和辣椒早疫病病原菌Alternaria solani的毒力。结果显示,内生菌DEB-2菌株能显著抑制5种辣椒病原真菌菌丝生长和孢子萌发,菌丝尖端均有不同程度变粗、膨大、崩解或者菌丝分枝增多的现象;分生孢子及芽管产生畸形。DEB-2菌株发酵液对5种病原菌菌丝生长的抑制中浓度EC50值分别是28.5、76.2、80.7、79.9、72.0μL/mL;对辣椒疫病病原菌和早疫病病原菌分生孢子萌发的EC50分别为73.5μL/mL和68.7μL/mL。研究表明,DEB-2菌株能有效地同时抑制5种辣椒病原真菌菌丝生长和孢子萌发,对该5种辣椒真菌病原菌具有一定的生防潜力,可作为生防菌进行开发和应用。
In order to explore the inhibitory effect of endophyte DEB-2 on the pepper fungal pathogens, the virulence of endophyte DEB-2 against five fungal pathogens of pepper from Guizhou was tested in the laboratory by mycelial growth inhibition method and spore germination inhibition method. The results showed that endophyte DEB-2 demonstrated strong virulence against five pathogens,including Alternaria alternate,Phytophthora capsici,Colletotrichum capsici,Colletotrichum gloesporioide and Alternaria solani. Morphological abnormalities of the inhibited hyphal tips caused by the isolate DEB-2 include bulges, thickening,collapse or swelling and stimulation of branching. The spores and their germ tubes became malformations. The EC50values of DEB-2 fermented liquid inhibiting mycelial growth of five pathogens were 28. 5,76. 2,80. 7,79. 9 and 72. 0 μL / mL,respectively. The EC50values of DEB-2 fermentedliquid inhibiting spore germination of P. capsici and A. solani were 73. 5 μL / mL and 68. 7 μL / mL, respectively. In conclusion,endophytic strain DEB-2 has great potential for biocontrol of those five fungal diseases of pepper.
出处
《植物保护学报》
CAS
CSCD
北大核心
2014年第2期157-162,共6页
Journal of Plant Protection
基金
贵州省农业攻关项目(黔科合NY字[2011]3034)
