摘要
我国葡萄品种资源丰富,栽培面积逐年增加。葡萄感染病毒后,便终生带毒,持续危害,造成生长发育迟缓,树势衰退,严重影响葡萄的产量和品质。山葡萄(Vitis amurensis Rupr.)是我国重要的野生果树资源,其抗病能力较强,是培育抗病、抗寒优良品种的珍贵种质。
Grapevine leafroll-associated virus 13( GLRaV-13) was reported in Japan in 2016 as a new member of genus Ampelovirus,and have not yet been found in other countries. In this study,we adopted small RNA sequencing to detect potential viruses in an amur grape cultivar“ZuoshanⅡ”exhibiting chlorotic mottling. By assembling the obtained small RNAs,112 contig sequences showed 87.5%-100% nucleotide identity and 64.9% coverage with the Japanese GLRaV-13 isolate a177 ( GenBank ID: NC_029783) . Two sets of primers,designed from the contig sequences,were used to amplify the cp and hsp70h gene sequences of the GLRaV-13 isolate, and the sequencing results showed the obtained sequences were the specific fragments of GLRaV-13. In addition, thirty amur grapevines and 90 other grapevines were used to detect GLRaV-13 using two pairs of primers,and the results showed that only amur grapevines tested positive for GLRaV-13. This is the first report of GLRaV-13 in China,and the study could help improve the sanitary status of grape cultivars in China.
作者
范旭东
张尊平
任芳
胡国君
董雅凤
FAN XU-dong;ZHANG Zun-ping;REN Fang;HU Guo-jun;DONG Ya-feng(National Center for Eliminating Viruses from Deciduous Fruit Tree,Research Institute of Pomology,Chinese Academy of Agriculture Sciences,Xingcheng 125100,China)
出处
《植物病理学报》
CAS
CSCD
北大核心
2019年第1期141-144,共4页
Acta Phytopathologica Sinica
基金
国家葡萄产业技术体系建设项目(CARS-29-bc-1)
关键词
病毒
葡萄
卷叶
抗病
资源
树势
种质
Grapevine leafroll-associated virus 13 ( GLRaV-13 )
small RNA sequencing
detection
sequence analysis
