摘要
基于33年的长期定位施肥试验,利用蛋白质组学的方法研究了长期定位施用高低氮肥对夏玉米(Zea mays)籽粒清蛋白的影响。试验共设3个处理:高氮组(年施用量为276kg·hm-2)、低氮组(年施用量为138kg·hm-2)以及对照组(不施氮肥)。以玉米品种‘鲁玉16’为材料,人工授粉后,在玉米成熟期取其穗中部籽粒,利用蛋白质双向电泳和质谱技术对籽粒清蛋白进行差异分析和鉴定。结果表明:与不施氮肥相比,长期高、低氮处理均导致清蛋白的含量降低,且在低氮条件下达显著水平。双向电泳结果表明长期低氮处理与不施氮肥相比共有7个差异蛋白点, 1个上调, 6个下调;长期高氮处理与不施氮肥相比共有6个差异蛋白点, 1个上调, 5个下调。差异蛋白的基因表达分析与双向电泳结果相一致。通过质谱鉴定并对其进行功能分类,共分为4类,分别为:蛋白质及氨基酸代谢相关蛋白、能量代谢相关蛋白、表达调控及防御抗逆相关的蛋白和功能未知蛋白。
The effects of high and low nitrogen fertilizers(276 and 138 kg·hm-2 per year,respectively)on albumin of summer maize(Zea mays)kernel were studied using proteomic method based on a long-term fertilization trial for 33 years.The middle grains were obtained from a maize variety‘Luyu 16’at the mature period after artificial pollination.Then,differentially expressed proteins were analyzed and identified using protein two-dimensional gel electrophoresis and mass spectrometry.The results showed that the content of albumin in the low nitrogen group significantly decreased compared to the control.A total of 7 and 6 differentially expressed proteins were identified in low and high nitrogen group,respectively.Moreover,compared to the control,6 and 5 proteins were down-regulated in low and high nitrogen group,respectively.The results of gene expression of different proteins were consistent with that of the two-dimensional electrophoresis.These identified proteins were subsequently classified into four categories according to their functions including protein and amino acid metabolism,energy metabolism,expression regulation and defense resistance as well as unknown function proteins.
作者
吴倩
许莹莹
刘树堂
裴玉贺
宋希云
WU Qian;XU Ying-Ying;LIU Shu-Tang;PEI Yu-He;SONG Xi-Yun(College of Agronomy,Qingdao Key Lab of Germplasm Innovation and Application of Major Crops,Qingdao Agricultural University,Qingdao,Shandong 266109,China;Beijing Pingchuan Jiaheng Technology Co.,Ltd.,Jinan 250000,China)
出处
《植物生理学报》
CAS
CSCD
北大核心
2019年第6期858-866,共9页
Plant Physiology Journal
基金
山东省农业良种工程项目(2017LZGC005)
山东省现代农业产业技术体系玉米产业创新团队项目(SDAIT-02-01)~~
关键词
长期定位施肥
氮肥
夏玉米
清蛋白
蛋白质组学分析
long-term located fertilization
nitrogen fertilizer
summer maize
albumin
proteomic analysis
