摘要
三胺酸环延伸(TALE)蛋白是一类在植物生长发育过程中调控分生组织分化的转录因子。本研究通过生物信息学手段从豆科模式植物百脉根(Lotus japonicus(Regel)K.Larsen)全基因组中筛选出分布于6条染色体上的40条TALE家族基因,并对其保守结构域、基因结构、系统进化、在染色体上的分布、理化性质以及部分典型基因的组织表达差异等进行分析。根据结构域不同可将百脉根TALE家族分为BELL和KNOX两个亚族;百脉根TALE家族在进化上较为保守,分化上与大豆存在较大差异;该家族基因有外显子4～6个,氨基酸序列长度在271～792之间,家族成员蛋白均为弱酸性蛋白。Realtime PCR分析表明该家族基因表达与motif元件数之间存在相关性;BELL亚族主要在顶芽表达,KNOX亚族则主要在根组织中表达。研究结果为进一步克隆百脉根TALE基因和分析其功能奠定基础。
TALE(three-amino acid-loop-extension)are essential transcription factors that regulate the differentiation of meristem in the plant growth and development.In this study,a total of 40 TALE family genes on six chromosomes were identified by bioinformatical analysis of the whole genome of the Leguminosae model plant Lotus japonicus(Regel)K.Larsen.Their conserved domain,gene structure,family evolution,chromosome distribution,physicochemical properties and expression differences were examined.As a result of the domain differences revealed that two major groups were divided into BELL and KNOX.Phylogenetic analysis found that the Lotus japonicus(Regel)K.Larsen TALE family gene sequences was conservative in evolution,while Soybean TALE gene was distinctly different with Lotus japonicus(Regel)K.Larsen in differentiation.The results of physicochemical properties showed their mainly possessed 4-6 exons encoding weak acid proteins of 271-792.The Real-time PCR result showed that TALE genes are diverse and exhibited different expression patterns in different tissues of Lotus japonicus(Regel)K.Larsen,related to the number of motif elements.BELL subfamily is highly expressed in the terminal bud,while KNOX subfamily is Prominent in the root.This study lays a foundation for further study on the complicated biological functions of Lotus japonicus(Regel)K.Larsen TALE.
作者
丘日光
张天缘
杨仕梅
宋莉
赵德刚
QIU Ri-guang;ZHANG Tian-yuan;YANG Shi-mei;SONG Li;ZHAO De-gang(College of Life Sciences and Institute of Agro-Bioengineering,Guizhou University/The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education)/Guizhou Key Lab of Agro-Bioengineering,Guiyang 550025;Guizhou Academy of Agricultural Sciences,Guiyang 550006)
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2019年第2期466-475,共10页
Journal of Plant Genetic Resources
基金
国家转基因生物新品种培育科技重大专项(2016ZX08010003)
国家自然科学基金项目(31660685)
贵州省科技计划项目(黔科合基础[2017]1119)~~
关键词
百脉根
TALE转录因子家族
生物信息学
系统进化
基因表达
Lotus japonicus(Regel)K.Larsen
TALE transcription factor family
bioinformatics
phylogenetic analysis
gene expression
