摘要
目的了解新生儿脐血IL-4、CD4+、CD8+CD4+/CD8+与乙型肝炎病毒(HBV)宫内感染的关联性,为早期发现新生儿感染HBV提供帮助。方法选择HBV感染的孕妇分娩的新生儿75例作为研究组,根据定性测得的乙肝两对半如乙肝表面抗原(HBSAg)、乙肝表面抗体(抗-HBS)、e抗原(HBeAg)、e抗体(抗-HBe)、核心抗体(抗-HBC)结果和定量测得的HBV-DNA值结果将研究组分为HBV宫内感染组17例,非宫内感染58例;并随机选择同一时间段内体检未发现异常的孕妇娩出的新生儿43例作为对照组,并使用不同的方法如酶联免疫吸附测定(ELISA)法检测IL-4水平、流式细胞术检测研究指标CD4+、CD8+、CD4+/CD8+各数值水平。分析3组新生儿脐血IL-4、CD4+、CD8+、CD4+/CD8+与HBV宫内感染的的相关性。结果 3组新生儿进行两两比较,宫内感染组脐血IL-4、CD4+、CD8+、CD4+/CD8+与非宫内感染组相比,宫内感染组新生儿脐血II-4、CD4+、CD8+、CD4+/CD8+与对照组相比,差异均有统计学意义(均P<0.05),而非宫内感染组新生儿脐血IL-4、CD4+、CD8+、CD4+/CD8+与对照组比较,差异均无统计学意义(均P>0.05)。宫内感染组新生儿IL-4、CD8+明显高于非宫内感染组,CD4+、CD4+/CD8+明显低于非宫内感染组,差异均有统计学意义(均P<0.05)。结论 HBV感染的孕妇分娩的新生儿脐静脉血IL-4水平升高,CD4+T细胞因子下降,CD8+T细胞因子升高,CD4+/CD8+比值下降,均有助于HBV宫内感染的早期发现和诊断。
Objective To study the neonatal umbilical cord blood IL-4,CD4+,CD8+,CD4+/CD8+and the relevance of hepatitis b virus(HBV)intrauterine infection and provide help for the early diagnosis of neonatal HBV infection.Methods Choose 75 cases with neonatal HBV infected pregnant childbirth as a team,according to the qualitative determination of second liver two half-and-half,such as hepatitis b surface antigen(HBSAg),hepatitis b surface antibody(anti-HBS),e antigen(HBeAg),e antibody(anti-HBe),core antibody(anti-HBC)and quantitative determination of HBV-DNA values will be the team divided into HBV intrauterine infection group of 17 cases,58 cases of intrauterine infection;not occurring And to choose a sample of a healthy pregnant woman in the same time period 43 of the newborns in the control group,and to use a different method like the enzyme link immunosorption(ELISA)method to determine the rate of the il-4,the flow cytometry of the CD4+,CD8+,CD4+/CD8+,the Numbers of the index.The correlation between i1-4,CD4+,CD8+,CD4+/CD8+and HBV intrauterine infection was analyzed.Results Three groups of newborns are compared,and two intrauterine infection group of umbilical cord blood IL-4,CD4+,CD8+,CP4+/CD8+compared with the intrauterine infection group,intrauterine infection group of neonatal umbilical cord blood IL-4,CD4+,CD8+,CD4+/CD8+compared with control group,the differences were statistically significant(P<0.05),rather than the intrauterine infection group of neonatal umbilical cord blood IL-4,CD4+,CD8+,CD4+/CD8+and the control group there is no statistical significance(P>0.05).The neonatal il-4,the CD8+is significantly higher than the absence of an intrauterine infection,CD4+,CD4+/CD8+,which is significantly lower than the absence of an intrauterine infection,and the difference is statistically significant.Conclusion Neonatal umbilical cord blood immune factor IL-4 levels,and CD4+T cell factor,CD8+T cells factor increases,CD4+/CD8+ratio decreased,due to all help in the early detection and diagnosis of HBV intrauterine infection.
作者
任常军
徐瑞红
赵瑞
相淑雯
李贺嘉
郝玲
REN Chang-jun;XU Rui-hong;ZHAO Rui;XIANG Shu-wen;LI He-jia;HAO Ling(Department of Pediatric,The First Hospital of Hebei Medical University,Shijiazhuang 050000,China)
出处
《中国误诊学杂志》
CAS
2019年第2期66-69,共4页
Chinese Journal of Misdiagnostics
基金
河北省科技厅科研项目(152777176)
