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Special AT-rich sequence-binding protein 2 acts as a negative regulator of stemness in colorectal cancer cells 被引量:5

Special AT-rich sequence-binding protein 2 acts as a negative regulator of stemness in colorectal cancer cells
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摘要 AIM To find the mechanisms by which special AT-rich sequence-binding protein 2(SATB2) influences colorectal cancer(CRC) metastasis.METHODS Cell growth assay, colony-forming assay, cell adhesion assay and cell migration assay were used to evaluate the biological characteristics of CRC cells with gain or loss of SATB2. Sphere formation assay was used to detect the self-renewal ability of CRC cells. The m RNA expression of stem cell markers in CRC cells with upregulated or downregulated SATB2 expression was detected by quantitative real-time polymerase chain reaction. Chromatin immunoprecipitation(Ch IP) was used to verify the binding loci of SATB2 on genomic sequences of stem cell markers. The Cancer Genome Atlas(TCGA) database and our clinical samples wereanalyzed to find the correlation between SATB2 and some key stem cell markers.RESULTS Downregulation of SATB2 led to an aggressive phenotype in SW480 and DLD-1 cells, which was characterized by increased migration and invasion abilities. Overexpression of SATB2 suppressed the migration and invasion abilities in SW480 and SW620 cells. Using sequential sphere formation assay to detect the selfrenewal abilities of CRC cells, we found more secondary sphere formation but not primary sphere formation in SW480 and DLD-1 cells after SATB2 expression was knocked down. Moreover, most markers for stem cells such as CD133, CD44, AXIN2, MEIS2 and NANOG were increased in cells with SATB2 knockdown and decreased in cells with SATB2 overexpression. Ch IP assay showed that SATB2 bound to regulatory elements of CD133, CD44, MEIS2 and AXIN2 genes. Using TCGA database and our clinical samples, we found that SATB2 was correlated with some key stem cell markers including CD44 and CD24 in clinical tissues of CRC patients.CONCLUSION SATB2 can directly bind to the regulatory elements in the genetic loci of several stem cell markers and consequently inhibit the progression of CRC by negatively regulating stemness of CRC cells. AIM To find the mechanisms by which special AT-rich sequence-binding protein 2(SATB2) influences colorectal cancer(CRC) metastasis.METHODS Cell growth assay, colony-forming assay, cell adhesion assay and cell migration assay were used to evaluate the biological characteristics of CRC cells with gain or loss of SATB2. Sphere formation assay was used to detect the self-renewal ability of CRC cells. The m RNA expression of stem cell markers in CRC cells with upregulated or downregulated SATB2 expression was detected by quantitative real-time polymerase chain reaction. Chromatin immunoprecipitation(Ch IP) was used to verify the binding loci of SATB2 on genomic sequences of stem cell markers. The Cancer Genome Atlas(TCGA) database and our clinical samples wereanalyzed to find the correlation between SATB2 and some key stem cell markers.RESULTS Downregulation of SATB2 led to an aggressive phenotype in SW480 and DLD-1 cells, which was characterized by increased migration and invasion abilities. Overexpression of SATB2 suppressed the migration and invasion abilities in SW480 and SW620 cells. Using sequential sphere formation assay to detect the selfrenewal abilities of CRC cells, we found more secondary sphere formation but not primary sphere formation in SW480 and DLD-1 cells after SATB2 expression was knocked down. Moreover, most markers for stem cells such as CD133, CD44, AXIN2, MEIS2 and NANOG were increased in cells with SATB2 knockdown and decreased in cells with SATB2 overexpression. Ch IP assay showed that SATB2 bound to regulatory elements of CD133, CD44, MEIS2 and AXIN2 genes. Using TCGA database and our clinical samples, we found that SATB2 was correlated with some key stem cell markers including CD44 and CD24 in clinical tissues of CRC patients.CONCLUSION SATB2 can directly bind to the regulatory elements in the genetic loci of several stem cell markers and consequently inhibit the progression of CRC by negatively regulating stemness of CRC cells.
出处 《World Journal of Gastroenterology》 SCIE CAS 2016年第38期8528-8539,共12页 世界胃肠病学杂志(英文版)
基金 Supported by National Natural Science Foundation of China,No.81525020,No.81502033,No.81272300 and No.31570753
关键词 SPECIAL AT-rich sequence-binding protein 2 Colorectal cancer STEMNESS Metastasis Special AT-rich sequence-binding protein 2 Colorectal cancer Stemness Metastasis
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  • 1Sheila K. Singh,Ian D. Clarke,Mizuhiko Terasaki,Victoria E. Bonn,Cynthia Hawkins,Jeremy Squire,Peter B. Dir.Identification of a Cancer Stem Cell in Human Brain Tumors. Cancer Research . 2003
  • 2Al-Hajj M,Wicha MS,Benito-Hernandez A,et al.Prospective identification of tumorigenic breast cancer cells. Proceedings of the National Academy of Sciences of the United States of America . 2003
  • 3Patani Neill,Jiang Wen,Mansel Robert,Newbold Robert,Mokbel Kefah.The mRNA expression of SATB1 and SATB2 in human breast cancer. Cancer Cell International . 2009
  • 4MV Zappone,R Galli,R Catena,N Meani,S De Biasi,E Mattei,C Tiveron,AL Vescovi,R Lovell-Badge,S Ottolenghi,SK Nicol.Sox2 regulatory sequences direct expression of a (beta)-geo transgene to telencephalic neural stem cells and precursors of the mouse embryo, revealing regionalization of gene expression in CNS stem cells. Development . 2000
  • 5Yang MH,Yu J,Jiang DM,et al.micro RNA-182 targets special AT-rich sequence-binding protein 2 to promote colorectal cancer proliferation and metastasis. Journal of Translational Medicine . 2014
  • 6Collins A T,Berry P A,Hyde C,et al.Prospective identification of tumorigenic prostate cancer stem cells[].Cancer Research.2005
  • 7A Eramo,F Lotti,G Sette,E Pilozzi,M Biffoni,A Di Virgilio,C Conticello,L Ruco,C Peschle,R De Maria.Identification and expansion of the tumorigenic lung cancer stem cell population. Cell Death and Differentiation . 2007
  • 8O’Brien CA,Pollett A,Gallinger S,et al.A human colon cancer cell capable of initiating tumour growth in immunodeficient mice[].Nature.2007
  • 9J Eberhard,A Gaber,S Wangefjord,B Nodin,M Uhlén,K Ericson Lindquist,K Jirstr?m.A cohort study of the prognostic and treatment predictive value of SATB2 expression in colorectal cancer. British Journal of Cancer . 2012
  • 10Xiaoying Zhao,Zhihu Qu,Jennifer Tickner,Jiake Xu,Kerong Dai,Xiaoling Zhang.??The Role of SATB2 in Skeletogenesis and Human Disease(J)Cytokine and Growth Factor Reviews . 2013

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