摘要
目的:探究电针预处理对动脉粥样硬化型兔腹腔巨噬细胞受体CD36及SR-A1的基因和蛋白表达的影响。方法:将32只雄性新西兰兔随机分为3组,即空白对照组(7只)、造模组(19只)、电针预处理组(6只)。兔动脉粥样硬化的模型通过高脂饲料喂养加行颈总动脉球囊损伤术。造模组再随机分为模型对照组、阳性药物组、电针治疗组,每组6只。造模组经8周造模成功后,进行4周的干预治疗。电针预处理组适应性喂养1周后,进行电针预处理,针刺双侧的内关穴、足三里穴以及关元穴,双侧内关穴、足三里穴连接电针仪20 min,采用疏密波(4 Hz/20 Hz,1 m A),每日1次,针刺6 d,休息1日,针刺3周后,再经8周造模,造模成功后,再给与电针治疗组相同的干预治疗。采用免疫组化法检测各组兔腹腔巨噬细胞受体CD36及SR-A1的蛋白表达情况;采用RT-PCR检测各组兔腹腔巨噬细胞受体CD36及SR-A1的基因表达情况。结果:与正常组比较:模型组的腹腔巨噬细胞受体CD36及SR-A1的基因和蛋白的表达均明显升高(P<0.05);与模型组比较:电针治疗组、阳性药物组的腹腔巨噬细胞受体CD36及SR-A1的基因和蛋白的表达均明显降低(P<0.05),电针治疗组与阳性药物组比较差异无统计学意义(均P>0.05);与电针治疗组、阳性药物组相比:电针预处理组的腹腔巨噬细胞受体CD36及SR-A1的基因和蛋白的表达均明显降低(P<0.05)。结论:电针预处理组能明显降低动脉粥样硬化兔腹腔巨噬细胞受体CD36及SR-A1的表达,这可能是针刺预防动脉粥样硬化疾病的机制之一。
Objective:This study has explored the effect of electroacupuncture preconditioning on gene and protein expression of atherosclerotic rabbit peritoneal macrophage receptor CD36 and SR-A1.Methods:Thirty-two male New Zealand rabbits were randomly divided into 3 groups:blank control group(7),modeling group(19),electroacupuncture pretreatment group(6).Atherosclerosis in rabbits was treated with high-fat diet and carotid artery balloon injury.The model group was further randomly divided into subgroups containing a model control group,a positive drug group,and an electroacupuncture treatment group with 6 rabbits in each group.The model group was given intervention treatment for 4 weeks after successful modeling for 8 weeks.After one week of adaptive feeding,the electro-acupuncture pretreatment group was treated with electro acupuncture,specifically.The acupuncture was given at Neiguan(PC6),Zusanli(ST36)and Guanyuan(RN4)respectively,bilateral Neiguan(PC6)and Zusanli(ST36),of which were connected with electro-acupuncture apparatus for 20 minutes.The density wave(4 Hz/20 Hz,1 m A)was implemented at a treatment by once a day,6 days continuous acupuncture,and 1 day rest.After 3 weeks acupuncture,a subsequent 8–week model building was tested.Finally,the same intervention treatment as the electroacupuncture treatment group was given after successful modeling.The protein expressions of CD36 and SR-A1 in peritoneal macrophages of rabbits in each group were detected by immunohistochemical method,and the gene expressions of CD36 and SR-A1 in peritoneal macrophages of rabbits in each group were detected by RT-PCR.Results:The mRNA and protein expression of peritoneal macrophage receptors CD36 and SR-A1 in the model group increased significantly(P<0.05)in comparison with the normal group.Additionally,the mRNA and protein expression of peritoneal macrophage receptors CD36 and SR-A1 in the electroacupuncture group and the positive drug group were significantly decreased(P<0.05)compared with the model group and the difference in the comparison between the electroacupuncture treatment group and the positive drug group was not statistically significant(all P>0.05).At last,compared with the electroacupuncture group and the positive group,the mRNA and protein expression of the peritoneal macrophage receptor CD36 and SR-A1 in the electroacupuncture preconditioning group were significantly decreased(P<0.05).Conclusion:Electroacupuncture preconditioning can significantly reduce the expression of peritoneal macrophage receptors CD36 and SR-A1 in atherosclerotic rabbits,which may be one of the mechanisms of acupuncture to prevent atherosclerotic diseases.
作者
章晓颖
王芮
王雅静
崔晨
张哲
贾连群
成泽东
ZHANG Xiaoying;WANG Rui;WANG Yajing;CUI Chen;ZHANG Ze;JIA Lianqun;CHENG Zedong(Liaoning University of Traditional Chinese Medicine,Shenyang 110847,Liaoning,China)
出处
《中华中医药学刊》
CAS
北大核心
2019年第6期1372-1376,1549-1550,共6页
Chinese Archives of Traditional Chinese Medicine
基金
国家自然科学基金项目(81674078)
辽宁省自然科学基金项目(201602479)
辽宁省高等学校创新人才支持计划项目(LR2016044)
沈阳市高层次创新人才计划项目(RC170333)
辽宁中医药大学中医脏象理论及应用教育部重点实验室开放基金项目(zyzx1708)
