摘要
目的优化补阳还五汤中总生物碱的提取工艺。方法用酸性染料比色法建立总生物碱含量的测定方法;采用L9（34）正交设计考察乙醇浓度、乙醇用量、提取次数等因素,以总生物碱含量为指标,优选总生物碱的提取工艺。结果补阳还五汤总生物碱的最佳工艺为:8倍量90%乙醇提取3次。结论优化的补阳还五汤总生物碱提取工艺条件,可为补阳还五汤方药的深入研究奠定基础,也可为相关制剂研究提供参考。
Objective: To optimize extraction process of alkaloid in Buyang Huanwu decoction. Methods: The determination of the content of total alkaloid was established with acid dye colorimetry method. With L_9(3~4) orthogonal design experiment,and by inspecting the extraction ethanol,ethanol concentration,extraction etc,the best extraction technology of total alkaloid in Buyang Huanwu decoction was selected. Results: The best process was 8 times the amount of 90% ethanol extracted for 3 times. Conclusion: Optimization of extraction process of alkaloid in Buyang Huanwu decoction can lay the foundation for the further study and provide references for the relevant development of preparations.
出处
《中国中医急症》
2016年第2期197-198,231,共3页
Journal of Emergency in Traditional Chinese Medicine
基金
国家自然科学基金(No.81202636
81274176)
浙江省自然科学基金(No.LZ14H270001)
浙江省中医药科技计划项目(No.2016ZZ2010)
关键词
补阳还五汤
总生物碱
正交设计
提取工艺
Buyang Huanwu decoction
Total alkaloid
Orthogonal design
Optimum extraction
