摘要
目的建立14批次半枝莲饮片标准汤剂质量评价方法,为半枝莲配方颗粒及其他半枝莲相关产品质量控制提供参考。方法依照标准汤剂的制备要求,制备14批标准汤剂,以野黄芩苷与野黄芩素为定量检测指标,计算出膏率与转移率,并建立超高效液相(UPLC)指纹图谱分析方法。结果 14批半枝莲标准汤剂中野黄芩苷与野黄芩素的转移率分别为23.0%~49.9%与14.2%~75.6%,出膏率为15.7%~21.8%。指纹图谱共有峰19个,指认4个,14批标准汤剂指纹图谱相似度均在0.9以上。结论建立了半枝莲标准汤剂的质量评价方法,质量标准完善,可为半枝莲配方颗粒质量控制方法提供参考。
Objective To establish a quality control method for standard decoction of Scutellaria barbata,and to provide reference for quality evaluation of Scutellaria barbata dispensing granules and other related products.Methods The standard decoction of 14 batches of Scutellaria barbata was prepared according to the standard decoction process.With scutellarin and scutellarein as the determination indexes,the transfer rates and extraction rates were calculated and the method of Ultra Performance Liquid Chromatography(UPLC)characteristic chromatograms analysis was established.Results According to the measurement of 14 batches of standard decoctions,the transfer rates of scutellarin and scutellarein ranged from 23.0%to 49.9%and 14.2%to 75.6%,and extraction rates were at the range of 15.7%-21.8%.Besides,the Similarity Evaluation System for Chromatographic Fingerprint of traditional Chinese medicine(TCM)was used to analyze and compare the characteristic chromatograms,19 common peaks were determined and 4 were calibrated.The similarity evaluation results of 14 batches of Scutellaria barbata standard decoction showed that the similarities were greater than 0.9.Conclusion The method for the quality evaluation of standard decoction of Scutellaria barbata was established in this study,which can provide a reference for the quality control of Scutellaria barbata formula granules.
作者
田宇柔
冯玉
麻景梅
王相
王鑫国
李军山
牛丽颖
TIAN Yurou;FENG Yu;MA Jingmei;WANG Xiang;WANG Xinguo;LI Junshan;NIULiying(Hebei University of Chinese Medicine,Shijiazhuang 050091 Hebei,China;Hebei TCM Formula Granule Engineering&Technology Research Center,Shijiazhuang 050091 Hebei,China;TCM Formula Granule Research Center of Hebei Province University,Shijiazhuang 050091 Hebei,China;Shineway Pharmaceutical Co.Ltd.,Shijiazhuang 051430 Hebei,China)
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2019年第7期851-857,共7页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
河北省高等学校科学技术研究资助项目(ZD2015001)
河北省中医药管理局科研计划项目(2017014)
关键词
半枝莲
野黄芩苷
标准汤剂
质量评价
指纹图谱
Scutellaria barbata
scutellarin
standard decoction
quality evaluation
fingerprint chromatogram
