摘要
目的探讨益气活血药(生脉注射液联合血塞通注射液)对心肌梗死大鼠心室缝隙连接蛋白43(Cx43)表达及室颤发生的影响。方法采用结扎心脏左冠状动脉前降支的方法建立心肌梗死大鼠模型,另设假手术(只穿线,不结扎)作对照。术后随机分为模型组、假手术组、卡托普利组和益气活血组。卡托普利组给予卡托普利片4.39 mg/(kg·d)溶解灌胃;益气活血组给予生脉注射液3.51mL/(kg·d),血塞通注射液17.54mg/(kg·d)腹腔注射;模型组和假手术组给予生理盐水,疗程4周。治疗结束后取材,采用real time PCR法检测左心室梗死边缘区Cx43mRNA的表达;Western blot法检测Cx43蛋白表达;在体电刺激记录室颤诱发率。结果与假手术组比较,模型组Cx43的mRNA和蛋白表达均显著降低(P<0.05或P<0.01);与模型组比较,卡托普利组和益气活血组Cx43的mRNA和蛋白表达均显著升高(P<0.05或P<0.01)。在体电刺激室颤诱发率模型组最高,卡托普利组和益气活血组的室颤诱发率降低,差异有统计学意义(P<0.05)。结论生脉注射液联合血塞通注射液可以减少心肌梗死后室颤的发生,其机制与改善心室Cx43表达有关。
Objective To study the effects of Yiqi Huoxue drugs(YQHX,combine Shengmai injection with Xuesaitong injection)on the expression of connexin 43(Cx43)and ventricular fibril ation in rats with myocardial infarction.Methods Model rats of myocardial in-farction were established by occlusion of the left anterior descending coronary artery,the sham operation group was without occlu-sion.After operation rats were randomly divided into sham operation group,model group,YQHX group and captopril group. YQHX [Shengmai injection 3.51 mL/(kg· d)and Xuesaitong injection 17.54 mg/(kg· d)]were administered intraperitoneal y.Captopril[4.39 mg/(kg· d)]and distil ed water (for the sham operation and model groups)were administered oral y.The course of treatment was 4 weeks.The expressions of Cx43 mRNA of myocardium in left ventricular infarct border zone were detected by real time fluorescent quantitative polymerase chain reaction after treatment.The expression of Cx43 protein was observed by western blot.The incidence of ventricular fibril ation was recorded by electric stimulation in vivo.Results Compared with sham operation group,the expressions of Cx43 mRNA and protein decreased remarkably in the model group (P<0.05 or P<0.01).Compared with model group,the expres-sions of Cx43 mRNA and protein increased significantly in YQHX group and captopril group (P<0.05 or P<0.01).The incidence of ventricular fibril ation was the highest in model group.The incidence of ventricular fibril ation reduced significantly in captopril group and YQHX group(P<0.05).Conclusion Shengmai injection and Xuesaitong injection can reduce the occurrence of ventricular fibril-lation after myocardial infarction.It is related to improving the expressions of Cx43.
出处
《中西医结合心脑血管病杂志》
2015年第16期1837-1839,共3页
Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease
基金
国家自然科学基金资助项目(No.81202685)
北京市教委科研基地项目(No.2012)
关键词
心肌梗死
心室颤动
缝隙连接蛋白43
益气活血
myocardial infarction
ventricular fibrillation
connexin 43
Yiqi Huoxue drugs
