摘要
采用紫外吸收光谱法对不同波长下油茶籽油质量浓度与吸光度的关系进行探讨,对油茶籽油质量浓度与吸光度的定量关系进行了研究。结果表明:当油茶籽油的质量浓度为1～10 mg/m L、0. 1～1 mg/m L、10～100μg/m L、1～10μg/m L时,测定波长分别取240、215、210、200 nm,此时吸光度与样品质量浓度具有良好的线性关系;最大吸收峰所对应的波长及吸光度均与样品质量浓度具有对数关系。同时,将此方法与传统混合油质量浓度的测定方法进行对比,发现所得到的测定结果没有显著差异。
The correlation between mass concentration of oil-tea camellia seed oil and ultraviolet absorbance under different wavelengths was discussed by ultraviolet absorption spectrometry,and the quantitative relationship between mass concentration of oil-tea camellia seed oil and ultraviolet absorbance was studied. The results showed that when the mass concentration of oil-tea camellia seed oil were 1-10 mg/mL,0. 1-1 mg/mL,10-100 μg/mL and 1-10 μg/mL,the determination wavelengths were 240,215,210 nm and 200 nm respectively,and there was a good linear relationship between sample mass concentration and absorbance. The wavelength and absorbance of maximum absorption peak had logarithmic relationship with sample mass concentration. At the same time,the method was compared with the traditional method of determination of mixed oil mass concentration,and there was no significant difference between the results of the two methods.
作者
黄三萍
权婷
肖新生
唐满生
欧光川
HUANG Sanping;QUAN Ting;XIAO Xinsheng;TANG Mansheng;OU Guangchuan(Key Laboratory of Comprehensive Utilization of Advantage Plants Resources in Southern Hunan,Department of Chemistry and Biological Engineering,Hunan University of Science and Engineering,Yongzhou 425199,Hunan,China)
出处
《中国油脂》
CAS
CSCD
北大核心
2019年第1期137-141,共5页
China Oils and Fats
基金
湖南省自然科学基金面上项目(2018JJ2143)
湘南优势植物资源综合利用湖南省重点实验室开放基金(XNZW17C10
XNZW17C08)
湖南省大学生研究性学习和创新性实验计划项目(湘教通[2018]255号)
国家自然科学基金项目(51772091)
关键词
油茶籽油
紫外吸收光谱法
定量分析
oil-tea camellia seed oil
ultraviolet absorption spectrometry
quantitative analysis
