摘要
米糠毛油普遍酸价较高,采用常规的脱酸方式存在炼耗高、预处理要求高、营养成分损失大、油品品质差等问题。酶法脱酸可以有效避免上述问题。采用固定化脂肪酶CALB对米糠毛油进行酶法脱酸研究,得到优化工艺条件为甘油与游离脂肪酸摩尔比1∶3、剪切速度25 m/s、剪切时间20min、加酶量50 g/kg、反应温度70℃、反应时间6 h、甘油中水分含量25%、真空度小于1 000 Pa、通氮气,在此条件下酯化率可达92. 5%。
Crude rice bran oil generally has high acid value,and the conventional deacidification methods have the problems of high refining consumption,high pretreatment requirement,high loss of nutrients and poor quality of oil. Enzymatic deacidification can effectively avoid these problems. The enzymatic deacidification process of crude rice bran oil with immobilized lipase CALB was studied. The enzymatic deacidification conditions were optimized as follows: molar ratio of glycerol to free fatty acid 1:3,shear speed 25 m/s,shear time 20 min,enzyme dosage 50 g/kg,reaction temperature 70 ℃,reaction time 6 h,water content in glycerol 25%,vacuum degree less than 1 000 Pa,adding nitrogen. Under the optimal conditions,the esterification rate was 92. 5%.
作者
赵晨伟
王勇
李明祺
李明
李磊
金青哲
ZHAO Chenwei;WANG Yong;LI Mingqi;LI Ming;LI Lei;JIN Qingzhe(School of Food Science and Technology,Jiangnan University,Wuxi 214122,Jiangsu,China;Willmar Biotechnology Research&Development Center Shanghai Co.,Ltd.,Shanghai 200137,China;Novozymes(China)Investment Co.,Ltd.,Beijing 100085,China)
出处
《中国油脂》
CAS
CSCD
北大核心
2019年第4期17-20,共4页
China Oils and Fats
关键词
米糠油
酶法脱酸
固定化脂肪酶CALB
rice bran oil
enzymatic deacidification
immobilized lipase CALB
