摘要
目的 :建立培养人鼻黏膜上皮 (HNE)细胞的纤毛分化模型。方法 :HNE细胞培养在覆盖Ⅰ型胶原凝胶的支持膜上 ,采用无血清培养液 ,行气液界面 (ALI)培养 ,用扫描电镜及图像分析技术对纤毛面积进行定量分析。结果 :液面下培养 2周的HNE细胞纤毛分化很差 (0 .31% ) ,而行ALI培养 2周的HNE细胞纤毛分化数量明显增加 (8.6 2 % ) ,差异有统计学意义 (P <0 .0 1)。 结论 :采用ALI培养 ,提供细胞生长的极性环境 。
Objective:In the present study, our aim was to develop a method to quantitative cell differentiation in cultures of human nasal epithelium (HNE) cells.Method: HNE cells were cultured on collagengel-coated membranes at an air-liquid interface (ALI) in hormone-and grouth factor-supplemented medium. The percentage of the culture surface covered with ciliated cells was estimated using scanning electron microscope and image analysis.Result:In present study, if an ALI was not esfablishecl and the cells were maintained in the submerged state, ciliated cell differentation was pool, the average ciliated surface area was 0.3% when cultures were submerged for fourteen days. When HNE cells cultures in ALI, average ciliated surface area was 8.6%.Conclusion:Our study demonstrated increased ciliogenesis in ALI.
出处
《临床耳鼻咽喉科杂志》
CAS
CSCD
北大核心
2004年第2期88-90,共3页
Journal of Clinical Otorhinolaryngology
关键词
细胞
培养的
鼻粘膜
纤毛
Cells,cultured
Nasal mucosa
Cilia
