摘要
目的:探讨叶酸、维生素B12及血浆同型半胱氨酸(Hcy)水平与血管性痴呆(vasculardementia,VD)的关系。方法:应用高效液相色谱仪和电化学检测法测定37例VD患者的血浆总Hcy水平,并与40例正常同龄对照组及40例非痴呆脑梗死组比较,运用聚合酶链反应-限制性内切酶片段长度多态性技术检测N5,N10-亚甲基四氢叶酸还原酶(methylenetetrahydrofolatereductase,MTH-FR)基因多态性,同时测定血浆叶酸及维生素B12水平。结果:VD患者血浆总Hcy水平犤(28.79±6.48)μmol/L犦显著高于非痴呆脑梗死组犤(25.34±5.36)μmol/L犦(t=2.553,P=0.005);非痴呆脑梗死组患者血浆总Hcy水平显著高于正常同龄对照组犤(19.71±2.82)μmol/L犦(t=16.322,P=0.0001)。MTHFR基因型有3种,即纯合子(T/T)型,杂合子(T/C)型,纯合子(C/C)型。3组基因型和等位基因频率相比差异均无显著性意义。VD组患者血浆叶酸及维生素B12水平明显低于非痴呆脑梗死组(t=2.329,3.275;P=0.026,0.001);非痴呆脑梗死组患者血浆叶酸及维生素B12水平明显低于正常同龄对照组(t=3.302,2.328;P=0.001,0.027)结论:高同型半胱氨酸血症可能是VD发病的一个新的危险因素。
AIM:To explore the relationship between folacin,vitamin B12 and plasma homocysteine(Hcy) levels in vascular dementia(VD). METHODS:37 VD patients,who were being cured,and 40 normal subjects of the same age as control group and 40 patients with non dementia cerebral infarction were enrolled in the study.Their plasma total Hcy levels were measured using high performance liquid chromatography and electrochemical detection.The polymorphisms of methylenetetrahydrofolate feductase(MTHFR) gene were analyzed by using polymerase chain reaction restriction fragment length polymorphism(PCR RFLP),plasma folate,serum Vitamin B12 were also determined. RESULTS:Total plasma homocysteine levels in the VD group[(28.79±6.48)μmol/L]were significantly higher than those in the non dementia cerebral infarction group[(25.34±5.36) μmol/L](t =2.553,P=0.005),which was significantly higher than that in the control group[(9.71±2.82) μmol/L](t=16.322,P=0.000 1).There were three MTHFR genotypes:T/T(homozygous mutation),T/C(heterozygous mutation) and C/C(wild type). There were no significant differences between the frequencies of the genotypes and alleles of MTHFR gene in three groups.Plasma folate serum Vitamin B12 levels in the AD group were significantly lower than those in the non dementia cerebral infarction group(t =2.329,3.275;P = 0.026,0.001),which were significantly lower than those in the control group(t =3.302,2.328;P = 0.001,0.027). CONCLUSION:Hyper homocysteinemia may be a new risk factor for the onset of VD.
出处
《中国临床康复》
CAS
CSCD
2003年第31期4256-4257,共2页
Chinese Journal of Clinical Rehabilitation
基金
博士后基金资助项目(LRB00071)~~
