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L型Ca^(2+)通道自发激活对静息心肌细胞钙火花的影响 被引量：1

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摘要 钙火花是心肌细胞肌浆网Ca^(2+)释放的基本单位。为了研究L型Ca^(2+)通道自发开放对心肌细胞钙火花的影响,实验使用激光共聚焦扫描显微镜和Ca^(2+)荧光探针Fluo-4,在大鼠心肌细胞上观察局部钙火花的发放。结果表明,0.2mmol/L CdCl2通过阻断L型Ca^(2+)通道,使自发性钙火花的发放频率从给药前的4.20下降到给药后的2.04个/(100μm·S),但不影响火花的时空特性。对Cd^(2+)敏感的钙火花进行分析,推测在静息膜电位下(-80mV),L型Ca^(2+)通道的开放概率约为10^(-5)。因此,在静息心肌细胞中,L型Ca^(2+)通道低频随机开放对自发性钙火花的产生及细胞钙稳态调节有重要影响。
作者 张广钦 付昱 阳冬梅 郝雪梅 白淑华 汤依群 E.G.Lakatta 程和平
机构地区 北京大学生物膜与膜生物工程国家重点实验室 中国药科大学药理研究室 Laboratory of Cardiovascular Sciences Laboratory of Cardiovascular Sciences
出处 《中国科学（C辑）》 CSCD 北大核心 2003年第5期468-472,共5页 Science in China(Series C)
基金 国家重点基础研究发展规划(批准号:G2000057002) 国家自然科学基金(批准号:30200088)
关键词 心肌细胞 钙火花 L型Ca^2+通道 自发激活 钙释放 激光共聚焦扫描显微镜 激活机制
分类号 R331 [医药卫生—人体生理学]
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参考文献12

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同被引文献24

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中国科学（C辑）
2003年 第5期

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