摘要
临界休克量内毒素血症小鼠表现出耸毛、萎糜、活动减少、反应迟钝、厌食和体重减轻等症状。存活者在第3日症状基本消失,此时作免疫系统检查可见:胸腺萎缩;白细胞总数、淋巴细胞数、粒细胞数以及全血白细胞吞噬指数均显著降低;总E花环％和活性花环％明显减少;T淋巴细胞对PHA的分化增殖反应减弱,特异和非特异免疫系统均受到损伤。此时再用灵敏度为1ng/ml的鲎试剂已不能检出血中有注入的内毒素存在,说明第3日检出的免疫系统各项变化是内毒素损伤作用的后效应。用半临界休克量内毒素或与内毒素等量的生理盐水未引起小鼠任何异常反应,提示内毒素损伤免疫系统有一阈量存在。
The symptoms of endotoxemic mice produced by critical shock-dose endotoxin included:Restless, hair standing vertically,movement decreasing,no appetite and body weight loss; most of which disappeared in third day after administration of endotoxin. Then,the changes of immunological system were as follows: total number of white blood cell,the number of lymphocytes and neutrophils and phagocytic index fell respectively; The thymus became atrophy; the percentage of total E-rosettes and active Erosettes decreased; the proliferative response of T-lymphocyte to nitogen(PHA)was inhibited. At this time,the exogenous endotoxin in blood stream had become negative detected by limulus agent with its sensitivity to be 0.1/ml. But all above changes would not be seen in the mice administed half of the critical shock-dose endotoxin or isovolumetric N. S. All these suggested that there was a threshold dose of endotoxin that could damage the immunological system.
关键词
内毒素血症
免疫
试验
endotoxemic
immune
limulus test
