期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

OxLDL直接诱导人外周血单核细胞表达致炎细胞因子mRNA 被引量：4

Oxidized low density lipoprotein induces the human peripheral blood monocyte expression of proinflammatory cytokines mRNA
下载PDF
导出
摘要 目的 :探讨OxLDL在动脉粥样硬化血管局部免疫病理损伤中的作用机制。方法 :采用地高辛标记的寡核苷酸探针原位杂交技术 ,观察OxLDL对人外周血单核细胞MCP 1、PDGF、bFGF、IL 10 4种致炎细胞因子mRNA表达的影响。结果 :OxLDL(10～ 30mg L)同人外周血单核细胞孵育 2 4小时后能够以剂量依赖性方式显著提高MCP 1、bFGF、PDGF、IL 10 4种致炎细胞因子mRNA表达 (均P <0 0 0 1)。结论 :OxLDL能以剂量依赖性方式直接刺激人外周血单核细胞分泌致炎细胞因子mRNA。 Objective:To investigate the function of oxidized low density lipoprotein(OxLDL) in the formation of atherosclerotic immuno-pathological lesions in the local blood vessel.Methods:With the Digoxenin-labeled Oligonucleotide-probes In Situ Hybridization,studied the effects of OxLDL on the mRNA expression of proinflammatory cytokines including MCP-1, bFGF, PDGF and IL-10 in the human peripheral blood monocyte. Results:Monocyte was significantly increased the mRNA expression of MCP-1, bFGF, PDGF and IL-10 in a dose-dependent manner after incubating with OxLDL(10～30 mg/L)for 24 hours(P<0.001).Conclusion:OxLDL can directly stimulate human peripheral blood monocytes to excrete proinflammatory cytokines mRNA in a dose-dependent manner.
作者 雷新军 马爱群 任冰稳 耿涛 张葳
机构地区 西安交通大学第一医院心血管内科
出处 《中国免疫学杂志》 CAS CSCD 北大核心 2004年第2期93-96,共4页 Chinese Journal of Immunology
基金 西安交通大学教育部教育振兴行动计划
关键词 细胞因子 单核细胞 脂蛋白 动脉粥样硬化 Cytokine Monocyte Lipoprotein Atherosclerosis
分类号 R363.21 [医药卫生—病理学]
  • 相关文献

参考文献13

  • 1[1]Jaber J, Murin J, Kinova S et al.The role of infection and inflammation in the pathogensis of atherosclerosis[J]. Vnitr Lek, 2002;48(7):657-666.
  • 2[2]Hakkinen T, Karkola K, Yla-Herttuala S. Macrophages, smooth muscle cells, endothelial cells, and T-cells expression CD40 and CD40L in fatty streaks and more advanced human atherosclerotic lesions. Colocalization with epitopes of oxidized low-density lipoprotein, scavenger receptor, and CD16(Fc gamma RⅢ)[J]. Virchows Arch, 2000;437(4):396-405.
  • 3[3]Hirano K, Yamashita S, Nakagawa Y et al. Expression of human scavenger receptor class B type I in cultured human monocyte-derived macrophages and atherosclerotic lesions[J]. Circ Res, 1999;85(1):108-116.
  • 4[4]Ares M P, Bengt K, Per E et al. Oxidized LDL induces transcription factor activator protein-1 but inhibits activation of nucler factor-κB in human vascular smooth muscle cells[J]. Arterioscler Thromb Vasc Biol,1995;15(10):1584-1590.
  • 5[5]Frosteg Ard J, Uifgren AK, Nyberg P et al. Cytokine expression in advanced human atherosclerotic plaques:dominance of pro-inflammatory (Th1)and macrophage-stimulating cytokines[J]. Atherosclerosis, 1999;145(1):33-34.
  • 6[6]Van Berkel T J, Van Eck I, Herijgers N et al. Scavenger receptor class A and B,their role in atherogenesis and the matabolism of modified LDLand HDL[J]. Ann N Y Acad Sci, 2000;902(5):113-126.
  • 7[7]Aiello R J, Bourassa P A, Lindsey S et al. Monocyte chemoattractant protein-1 accelerates atherosclerosis in apolipoprotein E-degicient mice[J]. Arterioscler Thromb Vasc Biol, 1999;19(6):1518-1525.
  • 8[8]Kim H H, Ha Hj, Kim S O et al. KR31372, a benzopyran derivative, inhibits oxidized LDL-stimulated proliferation and migration of vascular smooth muscle cells[J]. Fundam Clin Pharmacol, 2000;14(5):469- 476.
  • 9[9]Cucina A, Pagliei S, Borrelli V et al. Oxidized LDL(OxLDL)induces production of platelet derived growth factor AA(PDGF AA)from aortic smooth muscle cells[J]. Eur J Vasc Endovasc Surg,1998;16(3):197-202.
  • 10[10]Pinderski,Oslund L J, Hedrick C C et al. Interleukin-10 blocks atherosclerotic events in vitro and in vivo [see comments] [J]. Arterioscler Thromb Vasc Biol, 1999;19(12):2847-2853.

同被引文献42

引证文献4

二级引证文献35

中国免疫学杂志
2004年 第2期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部