摘要
对一株高产细胞表面植酸酶酵母突变株WZ 4菌的细胞植酸酶进行了研究。探讨了菌体生长与产酶的关系。结果表明 :菌体在培养的前期植酸酶酶活很低 ,培养 30h后酶活迅速增加 ,酶活在菌体生长的平衡期达到最大 ,该菌产植酸酶为非生长偶联型。在此基础了解WZ 4菌细胞植酸酶的性质 ,实验表明 :该酶的最适pH为 5 ,最适温度为 5 0℃ ,Km(以植酸钠为底物 )为0 666mmol L。通过磷对产酶影响因素的实验研究 ,初步得到WZ 4菌产植酸酶受控于培养基中的磷浓度的结论 ,即最大产酶磷浓度为 0 5mg 1 0 0ml,当磷浓度大于 1 0mg 1 0 0ml时产酶被完全阻遏。
A high-yield cell-surface phytase yeast was studied to build foundation for deep study. The first, the relation between the growth and phytase producing was studied, the result indicated that the phytase activity is very low during the prophase of the cultivation, after 30 hours, the phytase activity increased quickly, and the phytase activity became max in the stationary phase. The character of the cell phytase of WZ-4 was researched subsequently, the experiment showed that the cell phytase showed the optimum pH of 5, and that the cell phytase displayed an optimum temperature of 50℃ and a K m value for Na-phytate of 0\^66mmol/L. Besides, the effect of phosphate concentration on the phytase producing was studied, the research showed that the phytase production of WZ-4 was controlled by the phosphate concentration in the medium; the maximum production of phytase occurred in medium containing 0 5mg phosphorus per 100ml; when the concentration of phosphorus in the medium is more than 10mg phosphorus per 100ml, the phytase production was completely repressed.
出处
《中国生物工程杂志》
CAS
CSCD
2003年第12期107-110,共4页
China Biotechnology
基金
吉林大学基金项目 :2 0 0 3 2 5 3
