摘要
目的 建立新生猪急性低氧性心肌损害模型。方法 19只新生猪随机分成对照组(n =8)和窒息组 (n =11)。夹闭气管插管 10min建立新生猪窒息模型。窒息复苏后 6h测定血清中肌酸激酶心肌同工酶 (CK MB)和心肌钙蛋白T(cTnT)的含量 ;运用超声心动图检测各项心功能指标 ,包括左室射血分数 (LVEF)、右室射血分数 (RVEF)、二尖瓣及三尖瓣口舒张期血流EV/AV比值 (MVE/A ,TVE/A)、二尖瓣及三尖瓣环运动速度e/a比值 (MVe/a ,TVe/a)、左室等容舒张期 (LVIRT)以及二尖瓣和三尖瓣返流指数 (MR ,TR) ;对照组在相应时间点检测。实验结束后进行心肌损害病理组织学评分。结果 复苏后 6h ,窒息组CK MB和cTnT含量分别为 ( 4 2 3± 156)IU/L和 ( 0 85± 0 64) μg/L ,高于对照组 [( 2 13± 3 0 )IU/L和 ( 0 0 8± 0 0 2 ) μg/L ,P均 <0 0 1] ;LVEF、RVEF、TVE/A、MVe/a和TVe/a分别为 ( 59 6± 8 6) %、( 60 2± 7 1) %、0 79± 0 2 1、0 77± 0 12和 0 78± 0 19,均低于对照组 [分别为 ( 67 5± 6 9) %、( 68 8± 7 4) %、1 14± 0 16、1 19± 0 18和 1 0 3± 0 2 7,P均 <0 0 5] ;心肌损伤病理学评分为 2 82± 0 98,高于对照组 ( 0 3 8± 0 53 ,P <0 0 1) ,且左、右室之间评分无差异。结论 新生猪窒?
Objective To establish a piglet model of acute hypoxic myocardial impairment Methods Nineteen newborn piglets were divided into two groups: control group (CON, n=8) and asphyxia group (ASP, n=11) In ASP group, asphyxia was induced by disconnecting the animal from the ventilator and clamping the inhalation tubing for 10 minutes Six hours after cardiopulmonary resuscitation (CPR), the levels of serum creatine kinase MB (CK-MB) and cardiac troponin T (cTnT) were detected Cardiac functions were evaluated by echocardiography Parameters included left ventricular ejection fraction (LVEF), right ventricular ejection fraction (RVEF), ratio of peak E velocity and peak A velocity of mitral flow and tricuspid flow (MVE/A and TVE/A), ratio of peak evelocity and a velocity of mitral annulus motion and tricuspid annulus motion (MVe/a and TVe/a), isovolumic relaxation time of left ventricle (LVIRT), index of mitrial regurgitation and tricuspid regurgitation (MR and TR) The CON group was examined at the corresponding time points The pathologic changes in piglets′ hearts were examined The myocardial histopathologic damage score (MHDS) was used to evaluate the severity of myocardial impairments Results Six hours after CPR, the CK-MB and cTnT in ASP group [( 423±156) IU/L and(0 85±0 64)μg/L] were both higher than those in CON group[(213±30) IU/L and(0 08±0 02)μg/L, P< 0 01] The LVEF, RVEF, TVE/A, MVe/a and TVe/a in ASP group [(59 6±8 6)%, (60 2±7 1)%, 0 79±0 21, 0 77±0 12 and 0 78±0 19, respectively] were all lower than those in CON group[(67 5±6 9)%,(68 8±7 4)%, 1 14±0 16, 1 19±0 18 and 1 03±0 27,respectively, P< 0 05] The MHDS in ASP group was higher (2 82±0 98) than that in CON group (0 38±0 53, P< 0 01), but no significant difference was observed in MHDS between the left ventricle and right ventricle Conclusion Asphyxia can lead to obvious myocardial impairment in newborn piglet, which could be very useful for the studies of acute hypoxic myocardial impairment in the newborn infants
出处
《中华儿科杂志》
CAS
CSCD
北大核心
2003年第10期766-769,共4页
Chinese Journal of Pediatrics
基金
高等学校骨干教师资助计划 ( 2 0 0 0 6 6 )
