摘要
以影响褐环粘盖牛肝菌菌种分离成活率、初始菌丝萌发速度、菌丝生长状况为参照标准,通过对培养基、分离材料、分离环境、培养方法4个方面10种因素研究表明,培养基以制作到分离时间10d的MMN配方为好,材料以阴、晴天采集的中、幼子实体的菌肉、菌管最好,分离环境可以野外就地、可以室内接种箱,培养方法以分离后试管斜放效果最好。
Taking the survival rate of the isolation of strain,the speed of germination of the first hypha and the state of hypha'growth as the standard,ten factors concerning the culture medium,the isolating material, the isolating environment and the culture method were studied.It showed that the medium should be ten days old from making to isolating,the best contents was BOH,the best materials were pileus and tubuli of the middle and young fructification that were collected in sunny or cloudy days.The isolating environment might be the open air or the transfer box in room.The best isolating method was to put the test tube slantways after isolating.
出处
《林业科学研究》
CSCD
北大核心
2004年第1期66-71,共6页
Forest Research
基金
2001年浙江省丽水市科技局"牛肝菌菌根成因及仿生栽培技术研究"(编号:2001003)
