摘要
以 p130 0 (含潮霉素抗性标记基因 )和 p13W 8(含反义蜡质基因 )为供体DNA ,通过根癌农杆菌(Agrobacteriumtumefaciens) 介导的方法 ,对水稻品种寒丰B的幼胚愈伤组织进行共转化 ,获得 35份转基因植株。经PCR检测 ,有 5份检测到反义蜡质基因和潮霉素抗性基因共存。在其T1 代材料中 ,通过PCR检测获得2 4份只带有反义蜡质基因而无潮霉素标记基因的转基因植株。结果证明 :在共转化植株后代中 ,通过筛选可获得无抗性标记基因的水稻转基因植株。
Using p1300 (containing hpt gene) and p13W8 (containing anti-waxy gene) as donor DNA, the calli induced from immature embryos of rice Hanfen B as recipients were co-transformed mediated by Agrobacterium tumefaciens, and 35 transgenic plants were obtained. PCR assay of the transgenic plants indicated that the anti-waxy gene and the hpt gene had been integrated into their 5 plants. 24 marker-free (no hpt gene) transgenic plants with target gene (anti-waxy gene) had been obtained from the T 1 plants. The results showed that the co-transformation system of mediated by Agrobacterium is one of efficient method to generate transgenic plants of rice with marker-free.
出处
《上海农业学报》
CSCD
2004年第1期33-36,共4页
Acta Agriculturae Shanghai
基金
上海市科委科技攻关项目 (编号 :0 13 912 0 19)
上海市农业科学院青年科技骨干培养基金项目资助
