摘要
建立了一种简单且具有型特异性的产气荚膜梭菌的多重PCR诊断方法。结果表明,该PCR特异性好,只有产气荚膜梭菌呈现阳性,被检验的其他7种梭菌以及大肠杆菌、葡萄球菌和多杀性巴氏杆菌均为阴性。该PCR具有较高的敏感性,最小检测量为4.2×105CFU/mL,单个菌落稀释10倍后也能被检测出,适用于医院、防疫和兽医诊断等部门,具有重要的公共卫生意义。
A simple and type-specific multiplex PCR for Clostridium perfringens is described. It can distinguish type A, B, C, D and E of C. perfringens. The specific of the PCR is higher, only Clostridium perfringens show positive, other 7 Clostridium strains, E. Coli, Staphylococcus and Pasteurella multocida all show negative. The sensitivity of the PCR is to detect 4.2×10~5 CFU/mL or 10^(-1) dilution for a single colony. It can be applied to (detect the) C.perfringens in (hospital), epidemic prevention and veterinary diagnosis institutes. It has great signification to public health.
出处
《中国兽药杂志》
2004年第2期22-25,共4页
Chinese Journal of Veterinary Drug
