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P53蛋白与SV40大T抗原之间相互作用的研究 被引量：1

Studies on the Interaction of P53 and SV40 Large T
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摘要 为确定一种定量研究酵母体内蛋白质 -蛋白质之间相互作用的简便、快捷的方法 ,为抗癌小分子化合物药物筛选提供一条可行性途径。本文选择P5 3蛋白与SV4 0大T抗原为靶蛋白对 ,首先用酵母双杂交系统 (LiAc法质粒共转化酵母细胞 )方法定性证明了两者之间存在相互作用 ,然后通过α -半乳糖苷酶活力定量测定了相互作用力的大小 ,并确定了最佳酶活测定时间 ,并与 β-半乳糖苷酶活力测定进行了比较 ,认为α-半乳糖苷酶活力定量测定是研究蛋白质 -蛋白质间相互作用的一种更加简便快捷的方法。 To identify a convenient method of studying protein-protein interaction in Saccharomyces cerevisiae and find an optimal way for anti-cancer drug screening,Protein53 and Simian virus 40(SV40) are used target proteins in the paper.The interaction between Protein 53 and Simian virus 40(SV40) was qualitatively proved using LiAc-mediated yeast transformation (yeast two-hybrid system).Then the interaction of the two proteins was quantitatively proved by means of α-Gal assay and identified the optimal time of yeast growth of α-Gal assay.At the same time,The α-Gal and β-Gal assay method were compared and we think that α-Gal quantitative assay is a more convenient method than β-Gal assay at the aspect ofstudying the interaction of protein-protein.It is potentially used as drug screening of small molecular compounds.
作者 马海蓉 李维琪 曹旭
机构地区 中科院新疆理化技术研究所
出处 《生物技术》 CAS CSCD 2004年第1期16-19,共4页 Biotechnology
关键词 抗原 酵母双杂交系统 a-半乳糖苷酶活力测定 P53蛋白 SV40 相互作用 Protein 53 and Simian virus 40(SV40) Yeast two-hybrid system α-Gal assay β-Gal assay
分类号 Q71 [生物学—分子生物学] Q503 [生物学—生物化学]
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参考文献2

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二级参考文献11

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共引文献26

同被引文献22

  • 1高宁,胡宝成.酵母双杂交系统的发展及其衍生系统的比较[J].生物技术通讯,2006,17(3):421-424. 被引量：4
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  • 9Causier B,Davies B.Analysing protein-protein interactions with the yeast two-hybrid system.Plant Molecular Biology,2002,50(6):855-870.
  • 10Lin L,Shi Y H,Luo Z P,et al.Protein-protein interactions in two potyviruses using the yeasttwo-hybrid system.VirusResearch,2009,142(1-2):36-40.

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2004年 第1期

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