摘要
背景:神经生长因子(nervegrowthfactor,NGF)在神经元的生长、存活、分化、保护神经元的退行性改变及神经损伤的自我保护与修复中具有重要作用,目前NGF保护神经元的分子病理学机制还有许多需要深入探讨的。目的:观察脑缺血老年大鼠部分脑区及小脑内源性神经生长因子的变化。设计:完全随机对照实验研究。地点与材料:本研究的地点为广州军区广州总医院实验科。材料为36只SD大鼠2～2.5年龄,雌雄不限。干预:用夹闭两侧颈总动脉法建立不完全性脑缺血动物模型,将SD大鼠随机分成正常对照组(A组)、假手术组(B组)、缺血30min再灌注6h(C组)、12h(D组)、24h(E组)、48h(F组)、7d(G组)和14d(H组),用免疫组织化学ABC染色法检测部分脑区神经元及小脑NGF的表达。在透射电镜下观察各组神经元超微结构变化。主要观察指标:各组神经元NGF表达定性及定量观察。结果:除A、B组外,各组顶叶皮质神经元均有NGF表达,其中E组和G组中量表达,神经元数量分别为(58.4±9.18)mm2和(56.2±10.87)mm2;除A组、B组、F组和H组外各组海马均有少量表达,神经元数量C组为(28.8±6.42)mm2、D组(30.4±12.12)mm2、E组(24.2±5.18)mm2、G组(15.6±4.39)mm2;小脑均没有表达;再灌注超过48h组海马神经元和小脑蒲肯野细胞损伤较重,顶叶皮质神经元轻度水肿。
BACKGROUND:Nerve growth factor(NGF),as a specific protein, plays an important role in growth,life,differentiation of neurons and protecting degenerative changes of neurons and damaged neural repair.At present,NGF is more and more respected and needs further discussion.OBJECTIVE:To observe the changes of the endogenous NGF partial brain areas and cerebella of aged rats with cerebral ischemia.DESIGN:A completely randomized controlled trial. SETTING and MATERIALS:A total of 36 SD rats of either gender, aged 2-2.5 years, were enrolled in the study. The study was completed in the Department of Laboratory, General Hospital of Guangzhou Command.INTERVENTION:The rats were randomly divided into normal control group(Group A),sham-operation group(Group B),and groups receiving reperfusion for 6 h (Group C),12 h(Group D), 24 h(Group E),48 h(Group F),7 d(Group G) and 14 d(Group H) after cerebral ischemia for 30 min.Models of incomplete cerebral ischemia were induced by blocking bilateral common carotid arteries.The expression of NGF was examined by immunohistochemistry,and neuronal ultrastructural change was observed under transmission electron microscope(TEM) at different time points after reperfusion.MAIN OUTCOME MEASURE:Quantitative and qualitative observation of NGF expression in the neurons of all the groups.RESULTS:The results of immunohistochemistry showed that there was NGF expression in the parietal profile cortical neurons of all the groups except Group A and B.Of them,moderate amount of expression was showed in Group E and G,(quantity of neurons/mm2 were 58.4±9.18 and 56.2±10.87);small amount of expression in hippocampal neurons of all the groups except Group A,B,F,and H,and low level of expression in the frontal profile neurons was shown in all the groups(Group C 28.8±6.42,Group D 30.4±12.12,Group E 24.2±5.18 and Group G 15.6±4.39).There was nearly no expression of NGF in cerebella of all of groups.Ultrastructure of hippocampal neurons and Purkinje cells of the aged rats with ischemia/reperfusion over 48 h was damaged more severely.The parietal profile cortical neurons swelled lightly. CONCLUSION:Endogenous NGF play a neuroprotective role once the brain of aged rats was damaged.This study provides an experimental foundation for the clinical application of NGF in the treatment of ischemic cerebral damage.
出处
《中国临床康复》
CSCD
2004年第7期1351-1353,共3页
Chinese Journal of Clinical Rehabilitation
