摘要
介绍了一种在自制的聚砜中空纤维膜基础上改进接口的连接方法,并将其用于构建二维毛细管电泳蛋白质分离技术平台。经改进的中空纤维膜接口具有死体积小、传质效率高、分析速度快等优点,是目前多维毛细管电泳较为理想的接口。本文以细胞色素C为标样对二维毛细管等电聚焦和毛细管区带电泳的分离性能进行了考察,同时对肺癌细胞的分泌蛋白进行了分离分析。实验结果表明:与一维系统分离结果比较二维毛细管电泳在分辨率和峰容量方面都有较大的提高。
A modification of the interface for construction of two-dimensional capillary electrophoresis separation platform based on the self-made polysufone hollow hiber membrane was introduced. The modified interface provided small dead volume, convenience of column switch, fastness of transmission mass and quick analysis. The Chrome C sample and proteins excreted from lung cancer cells were selected to demonstrate the interface' s feasibility and resolving performance in 2D CE system. The 2D CE separation system was found to greatly increase the resolving power and overall peak capacity over those obtained for either dimension alone.
出处
《生命科学仪器》
2004年第1期11-15,共5页
Life Science Instruments
基金
国家重点基础研究规划项目(973
001 CB510202)
国家自然科学基金(20375040)
中科院大连化物所创新基金
关键词
聚砜
中空纤维膜接口
二维毛细管电泳
蛋白质分离平台
Polysufone
Hollow fiber membrane interface
Two-dimensional capillary electrophoresis
Protein Separation Platform.
