摘要
目的 观察慢性丙型肝炎患者与自然阴转者外周血HCV准种构成的变化规律。方法应用基因扩增、分子克隆和测序的方法 ,对未接受过治疗的 4例慢性丙型肝炎患者与 4例自然阴转者前后 10年血清中丙型肝炎病毒 (hepatitisCvirus,HCV)核心 (C)区与包膜 2 (E2 )区基因片段进行了序列分析及遗传进化关系比较。结果 4例慢性丙型肝炎患者 10年前后血清HCVC区和其中 2名患者E2区准种群体组内与组间遗传距离没有明显差异。与自然阴转者相比 ,慢性丙型肝炎患者外周血HCVC区和E2区准种群体组内平均遗传距离较大。在同一患者体内HCVE2区准种群体组内遗传距离比C区组内遗传距离大 ,且二者遗传距离大小之间存在相关关系 (rs=0 .80 95 )。结论 在丙型肝炎疾病的自然病程中HCV准种构成可长期保持稳定 ;HCV准种遗传变异度大小可能与丙型肝炎疾病的转归相关。
Objective To investigate the evolution of HCV quasispecies in persistent and self-limited infection patients. Methods Peripheral blood HCV preserved for 10 years from 8 naive patients were analyzed. HCV core and E2 genes were cloned and sequenced. Genetic distances of different isolates at different time were analyzed by phylip software. Results The genetic distances of HCV quasispecies were higher in persistent infection patients than that of self-limited patients. In each patient the genetic distance of HCV E2 region isolates were higher than that of HCV C region which has some relationship between each other. In the 4 persistent patients there exist no differences between within-group and between-group genetic distance in HCV C region and also exist no differences in HCV E2 region in 2 of the 4 persistent patients. Conclusions The diversity degree of HCV quasispecies may affect the outcome of hepatitis C. Research on HCV C and E2 region to study HCV quasispecies may obtain different results. The distribution of HCV quasispecies may be stable in hepatitis C natural history.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2003年第10期841-843,共3页
National Medical Journal of China
基金
国家"十五"科技攻关计划资助项目( 2 0 0 1BA70 5B0 6)
国家自然科学基金资助项目 ( 3 0 170 844 )
国家高技术研究发展计划基金资助项目 ( 2 0 0 2AA2 170 71)
国家重点基础研究发展规划基金资助项目 (G19990 5 410 6)
