摘要
研究了以碳源为限制性底物时金色链霉菌连续培养特性和动力学。实验结果表明,Streptomycesaureofaciens的比生长速率与限制性底物浓度的关系符合Monod方程,并得到动力学参数max=0.0649h-1和Ks=8.47gL-1。菌体和产物金霉素chlortetracycline的得率系数分别为1.866gg-1和2.580gg-1,维持系数为0.0051g(gh)-1。菌体和产物的最大产率为0.859g(Lh)-1和0.0299g(Lh)-1。菌体和产物对氧的得率系数分别为60.81gmol-1和64.33gmol-1,对氧的维持系数为0.3390mmol(gh)-1。磷氧比P/O等于2.77。呼吸商RQ与m呈线性正比关系,随着m的减小,碳源代谢流从酵解途径逐渐向戊糖途径增强。代谢流的这种迁移有利于产物金霉素chlortetracycline的合成。
The kinetics and characteristics of Streptomyces aureofaciens in continuous cultivation with carbon source used as a limited substrate were investigated. It is found that Monod Equation can express the relationship between the specific growth rate and the residual saccharide concentration, in which mmax=0.0649h-1 and Ks=8.47gL-1. The biomass and Chlortetracycline( CTC) yield coefficients against carbon source are 1.866gg-1 and 2.580gg-1, respectively, and the growth maintenance coefficient is 0.0051g(gh)-1. The maximum biomass yield and the CTC yield are 0.859g(Lh)-1 and 0.0299g(Lh)-1, respectively. The biomass and CTC yield coefficients against oxygen are 60.81gmol-1 and 64.33gmol-1, respectively, and the growth maintenance coefficient against oxygen is 0.339mmol(gh)-1 and the ratio of P/O is 2.77. The relationship between respiratory quotient RQ and m is linear. With m decreasing, the metabolic flux of carbon source shifts increasingly from glycolysis pathway to hexosephophate pathway. The shift of metabolic flux is favourable to Chlortetracycline biosynthesis.
出处
《高校化学工程学报》
EI
CAS
CSCD
北大核心
2004年第1期67-72,共6页
Journal of Chemical Engineering of Chinese Universities
基金
上海市曙光计划基金(01SG28)。
关键词
金色链霉菌
连续培养
动力学
金霉素
Streptomyces aureofaciens
continuous cultivation
kinetics
chlortetracycline
