摘要
对大白菜与萝卜3个杂交组合授粉后15天的幼胚进行了离体改良液体培养和固体培养,比较了两种培养方法的培养成活率。3个杂交组合的改良液体培养成活率分别为30.0％、22.2％和65.0％,而固体培养成活率分别为0％、8.0％和20.0％。改良液体培养成活率高,接种方便迅速,培养速度快,是一条高效快速培养远缘杂种幼胚的途径。本试验共获得21个株系159株杂种苗。植株形态介于双亲之间,个别性状倾向双亲一方。杂种F_1根尖细胞染色体数有3种类型,即19、29和38。
Studies on development in vitro of embryos in the crosses of three Chinese cabbage(Brossica campestris ssp. pekinensis ( Lour. ) (Olsson ) CY. '2079-3', 'Yuanda baotou qing' and Er niu xing' with one radish ( Ra-phanus sativus L. ) CY. 'Re bai lobu' were carried out in a liquid medium and on a solid medium. The embryo age was 15 days after pollination. When a liquid medium was employed, sterilized ovules were cut in half and introduced to the medium kept constant motion. It is unnecessary of picking up the minute embryos under a stereoscopic microscope. But the success rates of the three cross combinations were 30.0%, 22.2% and 65.0%, respectively, which were significantly higher than those of the solid-culture (0%, 8.0% and 20.0%, respectively). Twenty-one lines including 159 plants were obtained in the experiment. Morphological traits of F_1 hybrid plants intermediated to the parents. There were three types of chromosome number in root-tip cell of the hybrid plants, 19, 29 and 38.
出处
《园艺学报》
CAS
CSCD
北大核心
1992年第4期353-357,共5页
Acta Horticulturae Sinica
关键词
幼胚培养
大白菜
萝卜
属间杂种
In vitro
Embryo culture
Chinese cabbage
Radish
Intergeneric hybrid
