摘要
具有分化能力的烟草花粉植株叶片愈伤组织,经过13个月的继代培养,其分化能力随继代次数的增加而逐渐丧失。与此同时,单倍体细胞逐渐减少,产生大量多倍体和非整倍体细胞。发现不同激素水平的处理,对愈伤组织的分化和染色体数目变异的效应也不同。
With the procession of thirteen month's subculture, the differentiating ability of the callus, coming from tobacco anther-cultured-plant's leaf and having differentiating ability, was found to reduced gradually. Meanwhile the ratio of haploid cell in the callus dropped gradually, and a lot of polyploid and aneuploid cells appeared. It was observed that the treatments of different hormone concentrations had the different effects on the differentiation and chromosome variation of the callus.
出处
《云南农业大学学报》
CAS
CSCD
1992年第4期193-198,共6页
Journal of Yunnan Agricultural University
关键词
烟草
组织培养
染色体
变异
Tobacco Tissue culture Chromosome variation Differentiation
