摘要
目的:寻找一种注射用胸腺肽适用的辅料。方法:以三批同一批次的胸腺肽原料液为实验对象,通过 Folin-酚法和 T 细胞活性测定法-脱 E 受体法研究了三种辅料对胸腺肽多肽含量及活性的影响。结果:7%甘露醇作为辅料时使胸腺肽多肽含量测定显著增高,而右旋糖酐则不影响测定结果,三种辅料对胸腺肽活性均无显著影响。结论:最终选用4%右旋糖酐作为注射用胸腺肽的辅料。
Objective:To find an apposite stabilizer and excipient for Thymopetides.Meth- ods:4%dextran 40,7%mannitol and 2%dextran plus 7% mannitol were selected as the stabilizer and excipient of thy mopetides.And the influence caused by them was studied on assay of the con- tent of polypetide in Thymopetides by lowry method and on assa of the activity T cell—casting E antibody method.Result:7%mannitol made a significantly and remarkably higher content of polypeptide,wherease dextran had little influenece.And the stabilizer and excipient had no signifi- cantly remarkable influence on the activity of thymopetides.Conclusion:finally,4%dextran was determined as the optimal stabilizer and excipient by comparing.
出处
《黑龙江医药》
CAS
2004年第2期118-119,共2页
Heilongjiang Medicine journal
