摘要
目的 :为经皮冠状动脉介入治疗(PCI)术后再狭窄研究提供理想动物模型。方法 :采用颈动脉逆行入径 ,用球囊导管对中国白兔双侧髂动脉行内膜损伤术 ,后给予高脂饮食建立双侧髂动脉粥样硬化狭窄模型 ,在此基础上行血管成形术建立再狭窄模型。结果 :(1)饲养后1 ,3,6周的血清胆固醇和甘油三酯水平显著高于饲养前 (P<0.01)。(2)血管成形术前髂动脉平均狭窄度左侧为 (50.80±14.12) %,右侧为 (51.20±13.94) % ,两者差别无统计学意义 (P>0.05),血管成形术后5周再狭窄程度模型组为 (55.20±14.47) %。 (3)病理HE染色及Masson染色示内膜显著增厚 ,主要由泡沫细胞、平滑肌细胞构成 ,内弹力膜分离断裂 ,中膜平滑肌细胞迁移入内膜层。 (4)透射电镜示膜结构破坏 ,胞浆内可见大小不等的脂质空泡沉积融合 ,多量胶原纤维束状增生。结论 :本方法成功建立了动脉粥样硬化狭窄和再狭窄动物实验模型 ,且简单、易行 ,模型稳定 。
Objective:To study the animal model of restenosis for investigating and preventing restenosis after percutaenous coronary intervention(PCI). Methods:The model of atherosclerotic stenosis was created by the injury of endothelium of double iliac artery with balloon by carotid converse path,and the rabbit was fed with high cholesterol.The restenosis model was created.Results:(1)The levels of serum cholesterol and triglyceride were significantly lower before feeding compared with that after feeding(P<0.01).(2)The stenosis of left iliac artery was (50.80±14.12)%,and stenosis of right iliac artery was (51.20±13.94)% before angiplasty.There was no significant difference(P>0.05).The restenosis was (55.20±14.47)% five weeks after angioplasty.(3)The tunica intima became thick,and consisted of foam cells and smooth mucle cells by pathological sections.The internal elastic membrane was broken,and the smooth mucle cells moved into tunica intima from tunica media.(4)The membrane structure was destroyed,a lot of fatty vacuoles were deposit,and collagen fiber was hyperplastic in plasma by the examination of transmission election microscopy.Conclusion: The animal model of atherosclerosis and restenosis was successfully created.This method was simple,economical,and manageable,and the model was steady,credible,and easily repeated.
出处
《天津医药》
CAS
北大核心
2004年第3期154-156,F002,共4页
Tianjin Medical Journal
基金
国家自然科学基金项目资助课题 (项目编号 :30170233)
