摘要
目的 :观察乳腺浸润性导管癌中 8p2 2、11p15、17p13三个染色体区的杂合性缺失 (LOH)及其与临床病理参数之间的关系。 方法 :从石蜡包埋的存档标本中选取 34例肿瘤组织和其对应的自身正常对照组织 ,用PCR方法扩增位于此三个染色体区域的三个微卫星位点D8S136、D11S988、TP5 3,扩增产物用 6 %聚丙稀酰胺凝胶电泳 ,银染显色后进行LOH的判定。用免疫组化方法观察ER、PR、P5 3和c erBb 2在乳腺癌中的表达情况。 结果 :34例乳腺癌中有 12例 (35 .2 9% )D8S136位点发生LOH ,D11S988和TP5 3两个位点分别发现 5例 (14 .71% )LOH。D11S988和TP5 3两个位点的LOH与临床病理参数之间无明显相关性 ,而D8S136发生LOH的肿瘤平均直径明显高于LOH阴性的肿瘤 (P =0 .0 0 4 9)。 结论 :染色体 8p2 2区域有较高的LOH发生率 ,位于此区域的肿瘤抑制基因的失活可能与乳腺癌的迅速生长有关。
Objective:To investigate the LOH at regions on chromosomal arm 8p22,11p15,17p13 and its relationship with clinicopathological parameters. Methods:Thirty-four paraffin-embedded tumor and corresponding noncancerous tissues were analysed. PCR was used to amplified three microsatellite markers D8S136,D11S988 and TP53 located at these chromosomal regions. PCR products were electrophoresed on 6%polyacrylamide gel and detected using silver staining. The P53, c-erBb-2,PR,ER status were determined by immunohistochemistry. Results:Of the three markers we studied, D8S136 was detected LOH at a frequency of 12 in 34 tumors(35.29%). D11S988 and TP53 were detected LOH at a frequency of 5 in 34 tumors(14.71%). There were no obvious associations between LOH at D11S988、TP53 and clinicopathological parameters, but the tumors with LOH at D8S136 were significant larger than that without LOH(P=0.0049). Conclusion: Invasive ductal carcinoma of the breast has a frequent LOH on chromosome 8p22. The loss or inactivation of putative tumor suppressor genes on 8p22 may contribute to the excessive growth of the tumors.
出处
《医学研究生学报》
CAS
2004年第3期204-206,F002,共4页
Journal of Medical Postgraduates
关键词
乳腺癌
杂合性缺失
染色体
Breast cancer
Loss of heterozygosity
Chromosoma
