摘要
应用pUC118/pUC119载体,将一个从小麦(Triticum aestivum L.cv.asarce)基因文库中分离到的含高分子量(HMW)谷蛋白基因的重组子进行了亚克隆,并制作了它们的逐次缺失系列克隆。用Sanger双脱氧末端终止法测定了其中HMW谷蛋白结构基因及编码区上游约560bp的核苷酸顺序,全长3067bp,结构基因由830个密码子组成。基因内部没有内含子,与以前报道的一个小麦HMW谷蛋白基因具有很高的同源性,但其编码区内部却出现了一个TAA终止密码,这是否是一个假基因有待于进一步的研究。
Subclones of a wheat (Triticum aestivum L.cv.asarce) genomic recombinant containing high-molecular-weight (HMW) glutenin gene were constructed by using pUC118/pUC119 vectors, their successive shorter deletions were also prepared.The nucleotide sequence of about 560 bp upstream of initiation codon and total coding region was analysed by Sanger's dideo-xynucleotide chain termination method.It has 3067 bp which includes 830 codons and reveals high homology with a previously reported HMW glutenin gene.This gene contains no intron but a TAA termination codon within the coding region.Whether this is a silent gene or not merits further investigation.
基金
国家高技术研究发展计划(863计划)资助项目
关键词
小麦
高分子量
谷蛋白基因
结构
Wheat
High molecular weight glutenin gene
Nucleotide sequence
