摘要
目的 :建立用反相高效液相色谱法测定解毒万灵丸 (苍术、麻黄、石斛等 )中盐酸麻黄碱和盐酸伪麻黄碱含量的方法。方法 :采用C18柱 ,流动相为 0 .1%磷酸溶液 乙腈 (95∶5 ) ,流速为 1.0mL·min-1,紫外检测波长为 2 0 6nm。结果 :盐酸麻黄碱线性浓度范围 0 .0 2 0 2 8～ 1.0 14 μg ,相关系数 0 .9999,平均回收率 97.6 9% ,相对标准偏差RSD为 0 .9% (n =6 )。盐酸伪麻黄碱线性浓度范围 0 .0 2 2 5 4～ 1.12 7μg ,相关系数 0 .9998,平均回收率 98.4 0 % ,相对标准偏差RSD为 2 .3% (n=6 )。结论 :本法重复性好 ,定量准确 ,可作为该制剂的定量分析方法。
AIM: To establish the method for determining ephedrine hydrochloride and pseudoephedrine hydrochloride in Jieduwanling Pills (Rhizoma atractylodis,Herba ephedrae,Herba dendrobii,etc.)by RP-HPLC. METHODS : The Agilent Extend C_ 18 column with acetonitrile-0.1%H_3PO_4(5∶95)as the mobile phase was used. The flow rate was at 1.0 mL·min -1 and the detective wavelength was at 206 nm. RESULTS : The calibration curve was linear at a range of 0.02028 ～1.014μg for the ephedrine hydrochloride( r =0.9999). the average recovery was 97.69% and RSD was 0.9%( n =6). The calibration curve was linear at a range of 0.02254 ～ 1.127 μg for the pseudoephedrine hydrochloride( r =0.9998). The average recovery was 98.40% and RSD was 2.3%( n =6). CONCLUSION : The method is accurate with a good reproducibility and can be used as a quantitative analysis for this preparation.
出处
《中成药》
CAS
CSCD
北大核心
2004年第3期195-198,共4页
Chinese Traditional Patent Medicine
关键词
盐酸麻黄碱
盐酸伪麻黄碱
解毒万灵丸
HPLC
ephedrine hydrochloride
pseudoephedrine hydrochloride
Jieduwanling Pills
HPLC
