摘要
目的:探讨心肌Toll样受体4表达的随龄变化,寻找老化心肌损害的防治方法。 方法:以雌性成年(8～12周)及老龄BALB/C小鼠(20个月)为实验对象,用反转录-聚合酶链反应(Reverse transcription-polymerase chain reaction,RT-PCR)和免疫组化方法,对成年及老龄小鼠心肌Toll样受体4mRNA表达及蛋白表达进行检测。 结果:与成年小鼠相比,老龄小鼠心肌Toll样受体4mRNA及蛋白表达均明显降低,其TLR4/GAPDH比值分别为0.70和0.37。免疫组化研究结果显示,TLR4蛋白分布于心肌细胞,胞核未着色。 结论:随着机体老化,心肌TLR4表达降低,可能与老龄心肌对感染的易患性增加有关。
AIM: To investigate the age-related changes of Toll-like receptor 4(TLR4) expression, and to seek the method for prevention and cure of aging myocardium lesion.
METHODS: Female adult(8 - 12 w, n = 5) and elder (20m, n = 5) BALB/c mice were studied, their TLR4 mRNA and protein expression were measured by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry.
RESULTS: Compared with adult mice, TLR4 expression in hearts of elder BALB/c mice decreased significantly, the ratios of TLR4/GAPDH were 0. 70 and 0. 37, respectively. The results of immunohistochemistry showed that TLR4 protein distributed in myocardial cells, and nucleus was not pig-menting.
CONCLUSION: With the aging of the organ, the declining TLR4 expression may be related to the high susceptibility of infection in aging myocardium.
出处
《中国临床康复》
CSCD
2004年第9期1656-1657,共2页
Chinese Journal of Clinical Rehabilitation
基金
国家重点基础研究发展规划(973)部分赞助(G2000057004)~~
