摘要
本实验建立了稳定的动脉器官培养方法并以联胺作用于培养的动脉引发脂质过氧化反应,系统地观察硒、维生素E及人参皂甙对内皮细胞脂质过氧化损伤的保护作用。结果表明:联胺激发和促进了脂质过氧化反应引起脂质过氧化物含量增加,内皮细胞生物膜系统损伤及PGI_2合成功能降低。而预先用200 ppb硒、50μg/ml维生索E、10μg/ml人参皂甙作用于动脉,可抑制联胺引起的组织脂贡过氧化物含量升高,使内皮细胞生物膜系统损伤程度减轻并增加PGI_2合成能力。表明硒、维生素E、人参皂甙在一定程度上对内皮细胞过氧化损伤有保护作用,可能对动脉粥样硬化有防治作用。这为抗氧化剂特别是人参皂甙作为抗氧化药物在临床上合理应用提供一定的理论依据。
Organ culture system of aorta from New Zealand white rabbits was established, in which segment of aorta could be maintained in culture for more than 10 days. Segments were used for studies of peroxidative injuries of endothelial cells initiated by thiol-oxidizing agent, diamide and also for studies of protective action of antioxidants, Selenium, Vitamin E and Ginsenosides. Diamide enhanced lipid peroxides formation of the aorta. Lipid peroxidation might induce ultrastructural changes of endothelial cells including plasma membrane blebbing formation, swelling of mitochondria and vacuolization. With these morphologic changes PGI_2 synthesis of the aorta decreased. Administration of antioxidants reduced the concentration of lipid peroxides in the tissue, while 6-keto-PGF_(1α) content in the medium was increased and cells injury was alleviated. These results indicated that through the protection of endothelial cells against lipid peroxidation, antioxidants might play an important role in the retarding of atherogenetic process.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
1992年第5期467-470,共4页
Chinese Journal of Pathophysiology
关键词
动脉
内皮
抗氧剂
动脉粥样硬化
Arteries
Endothelium
Lipid peroxides
Antioxidants
Atherosclerosis
